Peptide-imprinted polymer microspheres prepared by precipitation polymerization using a single bi-functional monomer.

Keiichi Yoshimatsu, Jason LeJeune, David A Spivak, Lei Ye

Research output: Contribution to journalArticlepeer-review

35 Citations (SciVal)

Abstract

A single bi-functional monomer, N,O-bismethacryloyl ethanolamine (NOBE), was used in precipitation polymerization system to synthesize molecularly imprinted polymer (MIP) microspheres. Highly specific binding sites were obtained for N-terminal protected neuropeptides, Boc-Leu-enkephalin and Pyr-Leu-enkephalin. The use of NOBE allowed binding sites to be formed in polymer microspheres that are able to recognize target peptides through the consensus C-terminal sequence. The interesting molecular binding results suggest a new approach for peptide analysis combining in situ chemical modification with MIP recognition under non-aqueous conditions.
Original languageEnglish
Pages (from-to)719-724
JournalAnalyst
Volume134
Issue number4
DOIs
Publication statusPublished - 2009

Subject classification (UKÄ)

  • Biochemistry and Molecular Biology

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