Persistent organic pollutants have dose and CAG repeat length dependent effects on androgen receptor activity in vitro.

Christel Björk; Hannah Nenonen; Aleksander Giwercman; Ake Bergman; Lars Rylander; Yvonne Giwercman

doi:10.1016/j.reprotox.2011.06.075

Persistent organic pollutants have dose and CAG repeat length dependent effects on androgen receptor activity in vitro.

Christel Björk, Hannah Nenonen, Aleksander Giwercman, Ake Bergman, Lars Rylander, Yvonne Giwercman

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Abstract

Recently, the effect of exposure to persistent organic pollutants (POPs) on sperm concentration was only seen in men with a short androgen receptor (AR) gene CAG repeat. In order to investigate whether these effects could be observed also in vitro, we tested the impact of 2,2',4,4',5,5'-hexachlorobiphenyl (CB-153) and 1,1-bis-(4-chlorophenyl)-2,2-dichloroethene (4,4'-DDE) on 5Î±-dihydrotestosterone activated ARs containing 16, 22 and 28 CAG repeats, respectively. Single exposure to 4,4'-DDE had the most pronounced effect on the AR activity containing 16 CAG repeats, whereas 28 CAG was the most sensitive variant when a mixture of the two compounds was added. Thus, our in vitro results have confirmed the in vivo data indicating a CAG repeat length dependent effect of endocrine disrupters on the AR activity.

Original language	English
Pages (from-to)	293-297
Journal	Reproductive Toxicology
Volume	32
Issue number	3
DOIs	https://doi.org/10.1016/j.reprotox.2011.06.075
Publication status	Published - 2011

Subject classification (UKÄ)

Obstetrics, Gynecology and Reproductive Medicine

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10.1016/j.reprotox.2011.06.075

Elsevier__Bjork_et_al

http://www.ncbi.nlm.nih.gov/pubmed/21729750?dopt=Abstract

1 Doctoral Thesis (compilation)

Genetic, environmental and life-style effects on androgen receptor function
Björk, C., 2012, Lund University. 164 p.
Research output: Thesis › Doctoral Thesis (compilation)

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abstract = "Recently, the effect of exposure to persistent organic pollutants (POPs) on sperm concentration was only seen in men with a short androgen receptor (AR) gene CAG repeat. In order to investigate whether these effects could be observed also in vitro, we tested the impact of 2,2',4,4',5,5'-hexachlorobiphenyl (CB-153) and 1,1-bis-(4-chlorophenyl)-2,2-dichloroethene (4,4'-DDE) on 5{\^I}±-dihydrotestosterone activated ARs containing 16, 22 and 28 CAG repeats, respectively. Single exposure to 4,4'-DDE had the most pronounced effect on the AR activity containing 16 CAG repeats, whereas 28 CAG was the most sensitive variant when a mixture of the two compounds was added. Thus, our in vitro results have confirmed the in vivo data indicating a CAG repeat length dependent effect of endocrine disrupters on the AR activity.",

author = "Christel Bj{\"o}rk and Hannah Nenonen and Aleksander Giwercman and Ake Bergman and Lars Rylander and Yvonne Giwercman",

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doi = "10.1016/j.reprotox.2011.06.075",

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AU - Björk, Christel

AU - Nenonen, Hannah

AU - Giwercman, Aleksander

AU - Bergman, Ake

AU - Rylander, Lars

AU - Giwercman, Yvonne

PY - 2011

Y1 - 2011

N2 - Recently, the effect of exposure to persistent organic pollutants (POPs) on sperm concentration was only seen in men with a short androgen receptor (AR) gene CAG repeat. In order to investigate whether these effects could be observed also in vitro, we tested the impact of 2,2',4,4',5,5'-hexachlorobiphenyl (CB-153) and 1,1-bis-(4-chlorophenyl)-2,2-dichloroethene (4,4'-DDE) on 5Î±-dihydrotestosterone activated ARs containing 16, 22 and 28 CAG repeats, respectively. Single exposure to 4,4'-DDE had the most pronounced effect on the AR activity containing 16 CAG repeats, whereas 28 CAG was the most sensitive variant when a mixture of the two compounds was added. Thus, our in vitro results have confirmed the in vivo data indicating a CAG repeat length dependent effect of endocrine disrupters on the AR activity.

AB - Recently, the effect of exposure to persistent organic pollutants (POPs) on sperm concentration was only seen in men with a short androgen receptor (AR) gene CAG repeat. In order to investigate whether these effects could be observed also in vitro, we tested the impact of 2,2',4,4',5,5'-hexachlorobiphenyl (CB-153) and 1,1-bis-(4-chlorophenyl)-2,2-dichloroethene (4,4'-DDE) on 5Î±-dihydrotestosterone activated ARs containing 16, 22 and 28 CAG repeats, respectively. Single exposure to 4,4'-DDE had the most pronounced effect on the AR activity containing 16 CAG repeats, whereas 28 CAG was the most sensitive variant when a mixture of the two compounds was added. Thus, our in vitro results have confirmed the in vivo data indicating a CAG repeat length dependent effect of endocrine disrupters on the AR activity.

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DO - 10.1016/j.reprotox.2011.06.075

M3 - Article

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SP - 293

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JO - Reproductive Toxicology

JF - Reproductive Toxicology

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ER -

Persistent organic pollutants have dose and CAG repeat length dependent effects on androgen receptor activity in vitro.

Abstract

Subject classification (UKÄ)

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Research output

Genetic, environmental and life-style effects on androgen receptor function

Cite this