Phase equilibria of model milk membrane lipid systems.

Rianne Waninge; Tommy Nylander; Marie Paulsson; Björn Bergenståhl

doi:10.1016/S0009-3084(03)00070-7

Phase equilibria of model milk membrane lipid systems.

Rianne Waninge, Tommy Nylander, Marie Paulsson, Björn Bergenståhl

Physical Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The phase behaviour of mixtures of recombined milk membrane lipids dioleoylphosphatidylcholine (DOPC), sphingomyelin (SM), dioleoylphosphatidylethanolamine (DOPE), phosphatidylinositol (PI) and dioleoylphosphatidylserine (DOPS) in 60% water was examined as a function of temperature between 5 and 90 °C. The aim was to examine under which lipid composition the average properties turn from balanced over to hydrophobic. The phase boundaries were determined by small angle X-ray diffraction (SAXD) and differential scanning calorimetry (DSC). The lamellar phase was dominating in the DOPC/SM/DOPE system. The phase boundary for the reversed hexagonal phase was only observed at high DOPE content within the examined temperature interval. The anionic phospholipids PI and DOPS induced a swollen lamellar phase, but no significant change of the transition between the lamellar phase and the reversed hexagonal phase was observed.

Original language	English
Pages (from-to)	59-68
Journal	Chemistry and Physics of Lipids
Volume	125
Issue number	1
DOIs	https://doi.org/10.1016/S0009-3084(03)00070-7
Publication status	Published - 2003

Subject classification (UKÄ)

Other Engineering and Technologies
Physical Chemistry (including Surface- and Colloid Chemistry)

Free keywords

Small angle X-ray diffraction (SAXD)
Differential scanning calorimetry (DSC)
Phase behaviour
Milk fat globule membrane (MFGM)
Phospholipid bilayer

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10.1016/S0009-3084(03)00070-7

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title = "Phase equilibria of model milk membrane lipid systems.",

abstract = "The phase behaviour of mixtures of recombined milk membrane lipids dioleoylphosphatidylcholine (DOPC), sphingomyelin (SM), dioleoylphosphatidylethanolamine (DOPE), phosphatidylinositol (PI) and dioleoylphosphatidylserine (DOPS) in 60% water was examined as a function of temperature between 5 and 90 °C. The aim was to examine under which lipid composition the average properties turn from balanced over to hydrophobic. The phase boundaries were determined by small angle X-ray diffraction (SAXD) and differential scanning calorimetry (DSC). The lamellar phase was dominating in the DOPC/SM/DOPE system. The phase boundary for the reversed hexagonal phase was only observed at high DOPE content within the examined temperature interval. The anionic phospholipids PI and DOPS induced a swollen lamellar phase, but no significant change of the transition between the lamellar phase and the reversed hexagonal phase was observed.",

keywords = "Small angle X-ray diffraction (SAXD), Differential scanning calorimetry (DSC), Phase behaviour, Milk fat globule membrane (MFGM), Phospholipid bilayer",

author = "Rianne Waninge and Tommy Nylander and Marie Paulsson and Bj{\"o}rn Bergenst{\aa}hl",

year = "2003",

doi = "10.1016/S0009-3084(03)00070-7",

language = "English",

volume = "125",

pages = "59--68",

journal = "Chemistry and Physics of Lipids",

issn = "0009-3084",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Phase equilibria of model milk membrane lipid systems.

AU - Waninge, Rianne

AU - Nylander, Tommy

AU - Paulsson, Marie

AU - Bergenståhl, Björn

PY - 2003

Y1 - 2003

N2 - The phase behaviour of mixtures of recombined milk membrane lipids dioleoylphosphatidylcholine (DOPC), sphingomyelin (SM), dioleoylphosphatidylethanolamine (DOPE), phosphatidylinositol (PI) and dioleoylphosphatidylserine (DOPS) in 60% water was examined as a function of temperature between 5 and 90 °C. The aim was to examine under which lipid composition the average properties turn from balanced over to hydrophobic. The phase boundaries were determined by small angle X-ray diffraction (SAXD) and differential scanning calorimetry (DSC). The lamellar phase was dominating in the DOPC/SM/DOPE system. The phase boundary for the reversed hexagonal phase was only observed at high DOPE content within the examined temperature interval. The anionic phospholipids PI and DOPS induced a swollen lamellar phase, but no significant change of the transition between the lamellar phase and the reversed hexagonal phase was observed.

AB - The phase behaviour of mixtures of recombined milk membrane lipids dioleoylphosphatidylcholine (DOPC), sphingomyelin (SM), dioleoylphosphatidylethanolamine (DOPE), phosphatidylinositol (PI) and dioleoylphosphatidylserine (DOPS) in 60% water was examined as a function of temperature between 5 and 90 °C. The aim was to examine under which lipid composition the average properties turn from balanced over to hydrophobic. The phase boundaries were determined by small angle X-ray diffraction (SAXD) and differential scanning calorimetry (DSC). The lamellar phase was dominating in the DOPC/SM/DOPE system. The phase boundary for the reversed hexagonal phase was only observed at high DOPE content within the examined temperature interval. The anionic phospholipids PI and DOPS induced a swollen lamellar phase, but no significant change of the transition between the lamellar phase and the reversed hexagonal phase was observed.

KW - Small angle X-ray diffraction (SAXD)

KW - Differential scanning calorimetry (DSC)

KW - Phase behaviour

KW - Milk fat globule membrane (MFGM)

KW - Phospholipid bilayer

U2 - 10.1016/S0009-3084(03)00070-7

DO - 10.1016/S0009-3084(03)00070-7

M3 - Article

SN - 0009-3084

VL - 125

SP - 59

EP - 68

JO - Chemistry and Physics of Lipids

JF - Chemistry and Physics of Lipids

IS - 1

ER -

Phase equilibria of model milk membrane lipid systems.

Abstract

Subject classification (UKÄ)

Free keywords

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