Phenotypic protein profiling of different B cell sub-populations using antibody CD-microarrays

Peter Ellmark, Carl-Magnus Högerkorp, Sara Ek, Larissa Beelov, Mattias Berglund, Richard Rosenquist, Richard Christopherson, Carl Borrebaeck

Research output: Contribution to journalArticlepeer-review

Abstract

Antibody microarrays enable extensive protein expression profiling, and provide a valuable complement to DNA microarray-based gene expression profiling. In this study, we used DotScan™ antibody microarrays that contain antibodies against 82 different cell surface antigens, to determine phenotypic protein expression profiles for human B cell sub-populations. We then demonstrated that the B cell protein profile can be used to delineate the relationship between normal B cells and malignant counterparts. Principle component analysis showed that the lymphomas did not cluster with the normal memory B cells or germinal centre B cells, but they did cluster with germinal centre founder cells and naïve B cells.
Original languageEnglish
Pages (from-to)98-106
JournalCancer Letters
Volume265
DOIs
Publication statusPublished - 2008

Subject classification (UKÄ)

  • Cancer and Oncology

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