Phosphatidylethanol in blood as a marker of alcohol abuse

Arthur Varga

Phosphatidylethanol in blood as a marker of alcohol abuse

Arthur Varga

Associative Learning

Research output: Thesis › Doctoral Thesis (compilation)

Abstract

Biological markers offer a way of assessing ethanol intake and determining whether a health problem is alcohol related. This study investigated the use of phosphatidylethanol (PEth) in blood as a new marker of alcohol abuse. PEth is an abnormal phospholipid, formed only in the presence of ethanol via the transphosphatidylation reaction of phospholipase D (PLD). A new HPLC-evaporative light scattering detector (ELSD) method was developed for the analysis of PEth in blood. The method has high precision and analytical specificity with few interferents and a very high biological specificity. Inmoderate drinking (>50 g ethanol/day for several weeks) is required to give detectable PEth. A dose-response between alcohol intake and level of PEth seems to occur. PEth is almost completely compartmentalized in the red blood cell (RBC) fraction of human blood. Chronic alcoholics display a one-compartment elimination of PEth in blood, with a half-life of 4 days and a normalization that takes up to 14 days after end of drinking. PEth is formed in human RBC at physiologically relevant ethanol concentrations (50 mM), with an accumulation rate that is slower compared to rates in other tissues. RBC with high MCV from alcoholics seem to accumulate twice as much PEth compared to RBC with normal MCV from healthy control individuals. Increased PEth formation by phorbol ester TPA and 1-octanol in RBC indicate involvement of protein kinase C (PKC) in PLD-formation of PEth. Enzyme inhibitor studies indicate that PEth in human RBC is metabolized by phosphatidic acid phosphohydrolase. These results support the use of PEth in blood as a reliable long-time state marker of alcohol abuse.

Original language	English
Qualification	Doctor
Awarding Institution	Department of Laboratory Medicine
Supervisors/Advisors	[unknown], [unknown], Supervisor, External person
Award date	2001 Dec 11
Publisher	Dept of Medical Neurochemistry, Lund University Hospital, SE-221 85 Lund, Sweden.,
ISBN (Print)	91-628-5047-4
Publication status	Published - 2001

Bibliographical note

Defence details

Date: 2001-12-11
Time: 13:00
Place: Segerfalksalen WNC, BMC, Sölvegatan 17, Lund

External reviewer(s)

Name: Helander, Anders
Title: Associate Professor
Affiliation: Karolinska Hospital, Stockholm, Sweden

---

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Medical Neurochemistry (ceased) (LUR000012), Associative Learning (013212013)

Subject classification (UKÄ)

Neurology

Free keywords

PKC
MCV
ELSD
HPLC
red blood cell
phospholipase D
phosphatidylethanol
ethanol
alcohol abuse
state marker
Neurology
neuropsychology
neurophysiology
Neurologi
neuropsykologi
neurofysiologi

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Phosphatidylethanol in blood as a marker of alcohol abuse",

abstract = "Biological markers offer a way of assessing ethanol intake and determining whether a health problem is alcohol related. This study investigated the use of phosphatidylethanol (PEth) in blood as a new marker of alcohol abuse. PEth is an abnormal phospholipid, formed only in the presence of ethanol via the transphosphatidylation reaction of phospholipase D (PLD). A new HPLC-evaporative light scattering detector (ELSD) method was developed for the analysis of PEth in blood. The method has high precision and analytical specificity with few interferents and a very high biological specificity. Inmoderate drinking (>50 g ethanol/day for several weeks) is required to give detectable PEth. A dose-response between alcohol intake and level of PEth seems to occur. PEth is almost completely compartmentalized in the red blood cell (RBC) fraction of human blood. Chronic alcoholics display a one-compartment elimination of PEth in blood, with a half-life of 4 days and a normalization that takes up to 14 days after end of drinking. PEth is formed in human RBC at physiologically relevant ethanol concentrations (50 mM), with an accumulation rate that is slower compared to rates in other tissues. RBC with high MCV from alcoholics seem to accumulate twice as much PEth compared to RBC with normal MCV from healthy control individuals. Increased PEth formation by phorbol ester TPA and 1-octanol in RBC indicate involvement of protein kinase C (PKC) in PLD-formation of PEth. Enzyme inhibitor studies indicate that PEth in human RBC is metabolized by phosphatidic acid phosphohydrolase. These results support the use of PEth in blood as a reliable long-time state marker of alcohol abuse.",

keywords = "PKC, MCV, ELSD, HPLC, red blood cell, phospholipase D, phosphatidylethanol, ethanol, alcohol abuse, state marker, Neurology, neuropsychology, neurophysiology, Neurologi, neuropsykologi, neurofysiologi",

author = "Arthur Varga",

note = "Defence details Date: 2001-12-11 Time: 13:00 Place: Segerfalksalen WNC, BMC, S{\"o}lvegatan 17, Lund External reviewer(s) Name: Helander, Anders Title: Associate Professor Affiliation: Karolinska Hospital, Stockholm, Sweden --- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medical Neurochemistry (ceased) (LUR000012), Associative Learning (013212013)",

year = "2001",

language = "English",

isbn = "91-628-5047-4",

publisher = "Dept of Medical Neurochemistry, Lund University Hospital, SE-221 85 Lund, Sweden.,",

type = "Doctoral Thesis (compilation)",

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T1 - Phosphatidylethanol in blood as a marker of alcohol abuse

AU - Varga, Arthur

N1 - Defence details Date: 2001-12-11 Time: 13:00 Place: Segerfalksalen WNC, BMC, Sölvegatan 17, Lund External reviewer(s) Name: Helander, Anders Title: Associate Professor Affiliation: Karolinska Hospital, Stockholm, Sweden --- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medical Neurochemistry (ceased) (LUR000012), Associative Learning (013212013)

PY - 2001

Y1 - 2001

N2 - Biological markers offer a way of assessing ethanol intake and determining whether a health problem is alcohol related. This study investigated the use of phosphatidylethanol (PEth) in blood as a new marker of alcohol abuse. PEth is an abnormal phospholipid, formed only in the presence of ethanol via the transphosphatidylation reaction of phospholipase D (PLD). A new HPLC-evaporative light scattering detector (ELSD) method was developed for the analysis of PEth in blood. The method has high precision and analytical specificity with few interferents and a very high biological specificity. Inmoderate drinking (>50 g ethanol/day for several weeks) is required to give detectable PEth. A dose-response between alcohol intake and level of PEth seems to occur. PEth is almost completely compartmentalized in the red blood cell (RBC) fraction of human blood. Chronic alcoholics display a one-compartment elimination of PEth in blood, with a half-life of 4 days and a normalization that takes up to 14 days after end of drinking. PEth is formed in human RBC at physiologically relevant ethanol concentrations (50 mM), with an accumulation rate that is slower compared to rates in other tissues. RBC with high MCV from alcoholics seem to accumulate twice as much PEth compared to RBC with normal MCV from healthy control individuals. Increased PEth formation by phorbol ester TPA and 1-octanol in RBC indicate involvement of protein kinase C (PKC) in PLD-formation of PEth. Enzyme inhibitor studies indicate that PEth in human RBC is metabolized by phosphatidic acid phosphohydrolase. These results support the use of PEth in blood as a reliable long-time state marker of alcohol abuse.

AB - Biological markers offer a way of assessing ethanol intake and determining whether a health problem is alcohol related. This study investigated the use of phosphatidylethanol (PEth) in blood as a new marker of alcohol abuse. PEth is an abnormal phospholipid, formed only in the presence of ethanol via the transphosphatidylation reaction of phospholipase D (PLD). A new HPLC-evaporative light scattering detector (ELSD) method was developed for the analysis of PEth in blood. The method has high precision and analytical specificity with few interferents and a very high biological specificity. Inmoderate drinking (>50 g ethanol/day for several weeks) is required to give detectable PEth. A dose-response between alcohol intake and level of PEth seems to occur. PEth is almost completely compartmentalized in the red blood cell (RBC) fraction of human blood. Chronic alcoholics display a one-compartment elimination of PEth in blood, with a half-life of 4 days and a normalization that takes up to 14 days after end of drinking. PEth is formed in human RBC at physiologically relevant ethanol concentrations (50 mM), with an accumulation rate that is slower compared to rates in other tissues. RBC with high MCV from alcoholics seem to accumulate twice as much PEth compared to RBC with normal MCV from healthy control individuals. Increased PEth formation by phorbol ester TPA and 1-octanol in RBC indicate involvement of protein kinase C (PKC) in PLD-formation of PEth. Enzyme inhibitor studies indicate that PEth in human RBC is metabolized by phosphatidic acid phosphohydrolase. These results support the use of PEth in blood as a reliable long-time state marker of alcohol abuse.

KW - PKC

KW - MCV

KW - ELSD

KW - HPLC

KW - red blood cell

KW - phospholipase D

KW - phosphatidylethanol

KW - ethanol

KW - alcohol abuse

KW - state marker

KW - Neurology

KW - neuropsychology

KW - neurophysiology

KW - Neurologi

KW - neuropsykologi

KW - neurofysiologi

M3 - Doctoral Thesis (compilation)

SN - 91-628-5047-4

PB - Dept of Medical Neurochemistry, Lund University Hospital, SE-221 85 Lund, Sweden.,

ER -

Phosphatidylethanol in blood as a marker of alcohol abuse

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

UN SDGs

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Cite this