Phylogenetic analysis and substrate specificity of GH2 β-mannosidases from Aspergillus species.

Sumitha Krishnaswamyreddy; Anna Rosengren; Sylvia Klaubauf; Tejas Kulkarni; Eva Nordberg Karlsson; Ronald P de Vries; Henrik Stålbrand

doi:10.1016/j.febslet.2013.08.029

Phylogenetic analysis and substrate specificity of GH2 β-mannosidases from Aspergillus species.

Sumitha Krishnaswamyreddy, Anna Rosengren, Sylvia Klaubauf, Tejas Kulkarni, Eva Nordberg Karlsson, Ronald P de Vries, Henrik Stålbrand

Biochemistry and Structural Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised β-mannosidases from other organisms, clusters putative Aspergillus β-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched β-mannosides like mannobiose. A 3D-model of MndB provides further insight.

Original language	English
Pages (from-to)	3444-3449
Journal	FEBS Letters
Volume	587
Issue number	21
DOIs	https://doi.org/10.1016/j.febslet.2013.08.029
Publication status	Published - 2013

Subject classification (UKÄ)

Biological Sciences

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10.1016/j.febslet.2013.08.029

1 Doctoral Thesis (compilation)

β-Mannoside hydrolases from clan A – Structural enzymology, subsite engineering and transglycosylation
Rosengren, A., 2014, Department of Chemistry, Lund University. 180 p.
Research output: Thesis › Doctoral Thesis (compilation)

1 Finished

Galactomannan degradation by fungi and gut bacteria
Krishnaswamyreddy, S. (Researcher) & Stålbrand, H. (PI)
2011/01/01 → 2016/06/10
Project: Dissertation

Cite this

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title = "Phylogenetic analysis and substrate specificity of GH2 β-mannosidases from Aspergillus species.",

abstract = "Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised β-mannosidases from other organisms, clusters putative Aspergillus β-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched β-mannosides like mannobiose. A 3D-model of MndB provides further insight.",

author = "Sumitha Krishnaswamyreddy and Anna Rosengren and Sylvia Klaubauf and Tejas Kulkarni and {Nordberg Karlsson}, Eva and {de Vries}, {Ronald P} and Henrik St{\aa}lbrand",

year = "2013",

doi = "10.1016/j.febslet.2013.08.029",

language = "English",

volume = "587",

pages = "3444--3449",

journal = "FEBS Letters",

issn = "1873-3468",

publisher = "Wiley-Blackwell",

number = "21",

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TY - JOUR

T1 - Phylogenetic analysis and substrate specificity of GH2 β-mannosidases from Aspergillus species.

AU - Krishnaswamyreddy, Sumitha

AU - Rosengren, Anna

AU - Klaubauf, Sylvia

AU - Kulkarni, Tejas

AU - Nordberg Karlsson, Eva

AU - de Vries, Ronald P

AU - Stålbrand, Henrik

PY - 2013

Y1 - 2013

N2 - Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised β-mannosidases from other organisms, clusters putative Aspergillus β-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched β-mannosides like mannobiose. A 3D-model of MndB provides further insight.

AB - Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised β-mannosidases from other organisms, clusters putative Aspergillus β-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched β-mannosides like mannobiose. A 3D-model of MndB provides further insight.

U2 - 10.1016/j.febslet.2013.08.029

DO - 10.1016/j.febslet.2013.08.029

M3 - Article

C2 - 24021641

SN - 1873-3468

VL - 587

SP - 3444

EP - 3449

JO - FEBS Letters

JF - FEBS Letters

IS - 21

ER -

Phylogenetic analysis and substrate specificity of GH2 β-mannosidases from Aspergillus species.

Abstract

Subject classification (UKÄ)

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Research output

β-Mannoside hydrolases from clan A – Structural enzymology, subsite engineering and transglycosylation

Projects

Galactomannan degradation by fungi and gut bacteria

Cite this