Plasmodium falciparum: a simplified technique for obtaining singly infected erythrocytes

Manoj K Puthia, Kevin S W Tan

Research output: Contribution to journalArticlepeer-review


We report the development of a simple technique involving 15 ml polypropylene tubes and a rotatory incubator for obtaining erythrocytes singly infected with Plasmodium falciparum. This technique will be useful for cloning of the parasite. Our finding that P. falciparum merozoite invasion is inhibited during rotation suggests that this method may also be useful for the study of parasite-erythrocyte interactions under dynamic circulatory conditions.

Original languageEnglish
Pages (from-to)176-8
JournalParasitology Research
Issue number3
Publication statusPublished - 2005 Feb
Externally publishedYes

Free keywords

  • Animals
  • Culture Media
  • Erythrocytes/cytology
  • Humans
  • Malaria, Falciparum/parasitology
  • Parasitemia/parasitology
  • Parasitology/instrumentation
  • Plasmodium falciparum/growth & development
  • Polypropylenes


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