Abstract
A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a simple, safe lysis procedure, we detected the 419-base-pair sequence in samples containing few mycobacteria. Preliminary data suggested that this technique could be applied to clinical specimens for early and specific diagnosis of tuberculosis.
| Original language | English |
|---|---|
| Pages (from-to) | 2200-2204 |
| Journal | Journal of Clinical Microbiology |
| Volume | 28 |
| Issue number | 10 |
| Publication status | Published - 1990 |
Subject classification (UKÄ)
- Microbiology in the Medical Area
