Polymorphism in mtDNA control region of Mizo-Mongloid Breast Cancer samples as revealed by PCR-RFLP analysis

Souvik Ghatak; Doris Lallawmzuali; Subhajit Mukherjee; Lal Mawia; Jeremy L Pautu; Nachimuthu Senthil Kumar

doi:10.3109/19401736.2014.982627

Polymorphism in mtDNA control region of Mizo-Mongloid Breast Cancer samples as revealed by PCR-RFLP analysis

Souvik Ghatak, Doris Lallawmzuali, Subhajit Mukherjee, Lal Mawia, Jeremy L Pautu, Nachimuthu Senthil Kumar

Research output: Contribution to journal › Article › peer-review

Abstract

Mutations in mitochondrial D-loop region of DNA (mtDNA) may serve as a potential sensor for cellular DNA damage and marker for cancer development. We investigated the restriction fragment length polymorphism (RFLP) pattern of the D-loop region in the blood samples of breast cancer patients among Mizoram population. Significant differences were observed among breast cancer and healthy blood samples in the RFLP pattern using AluI, HaeIII and RsaI enzymes. Polymorphic information content (PIC - 0.258), band informativeness (∑Ib - 3.283) and marker index (MI - 0.006) were highest in the case of RsaI enzyme. Our data suggest that the RsaI polymorphic site in the mitochondrial control region is an informative marker for breast cancer development in Mizo population.

Original language	English
Pages (from-to)	2205-8
Journal	Mitochondrial DNA, Part A: DNA mapping, sequencing, and analysis
Volume	27
Issue number	3
DOIs	https://doi.org/10.3109/19401736.2014.982627
Publication status	Published - 2016 May
Externally published	Yes

Subject classification (UKÄ)

Cancer and Oncology

Free keywords

Biomarkers, Tumor/genetics
Breast Neoplasms/blood
DNA, Mitochondrial/genetics
Female
Genes, Mitochondrial
Genome/genetics
Genome, Mitochondrial/genetics
Humans
Mitochondria/genetics
Mongolia
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length/genetics
Sequence Analysis, DNA/methods
Whole Genome Sequencing/methods

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3109/19401736.2014.982627

Cite this

@article{4b50e7618f374e7da97f98760fa2c9f8,

title = "Polymorphism in mtDNA control region of Mizo-Mongloid Breast Cancer samples as revealed by PCR-RFLP analysis",

abstract = "Mutations in mitochondrial D-loop region of DNA (mtDNA) may serve as a potential sensor for cellular DNA damage and marker for cancer development. We investigated the restriction fragment length polymorphism (RFLP) pattern of the D-loop region in the blood samples of breast cancer patients among Mizoram population. Significant differences were observed among breast cancer and healthy blood samples in the RFLP pattern using AluI, HaeIII and RsaI enzymes. Polymorphic information content (PIC - 0.258), band informativeness (∑Ib - 3.283) and marker index (MI - 0.006) were highest in the case of RsaI enzyme. Our data suggest that the RsaI polymorphic site in the mitochondrial control region is an informative marker for breast cancer development in Mizo population.",

keywords = "Biomarkers, Tumor/genetics, Breast Neoplasms/blood, DNA, Mitochondrial/genetics, Female, Genes, Mitochondrial, Genome/genetics, Genome, Mitochondrial/genetics, Humans, Mitochondria/genetics, Mongolia, Polymerase Chain Reaction, Polymorphism, Genetic, Polymorphism, Restriction Fragment Length/genetics, Sequence Analysis, DNA/methods, Whole Genome Sequencing/methods",

author = "Souvik Ghatak and Doris Lallawmzuali and Subhajit Mukherjee and Lal Mawia and Pautu, {Jeremy L} and Kumar, {Nachimuthu Senthil}",

year = "2016",

month = may,

doi = "10.3109/19401736.2014.982627",

language = "English",

volume = "27",

pages = "2205--8",

journal = "Mitochondrial DNA, Part A: DNA mapping, sequencing, and analysis",

issn = "2470-1394",

publisher = "Taylor & Francis",

number = "3",

}

TY - JOUR

T1 - Polymorphism in mtDNA control region of Mizo-Mongloid Breast Cancer samples as revealed by PCR-RFLP analysis

AU - Ghatak, Souvik

AU - Lallawmzuali, Doris

AU - Mukherjee, Subhajit

AU - Mawia, Lal

AU - Pautu, Jeremy L

AU - Kumar, Nachimuthu Senthil

PY - 2016/5

Y1 - 2016/5

N2 - Mutations in mitochondrial D-loop region of DNA (mtDNA) may serve as a potential sensor for cellular DNA damage and marker for cancer development. We investigated the restriction fragment length polymorphism (RFLP) pattern of the D-loop region in the blood samples of breast cancer patients among Mizoram population. Significant differences were observed among breast cancer and healthy blood samples in the RFLP pattern using AluI, HaeIII and RsaI enzymes. Polymorphic information content (PIC - 0.258), band informativeness (∑Ib - 3.283) and marker index (MI - 0.006) were highest in the case of RsaI enzyme. Our data suggest that the RsaI polymorphic site in the mitochondrial control region is an informative marker for breast cancer development in Mizo population.

AB - Mutations in mitochondrial D-loop region of DNA (mtDNA) may serve as a potential sensor for cellular DNA damage and marker for cancer development. We investigated the restriction fragment length polymorphism (RFLP) pattern of the D-loop region in the blood samples of breast cancer patients among Mizoram population. Significant differences were observed among breast cancer and healthy blood samples in the RFLP pattern using AluI, HaeIII and RsaI enzymes. Polymorphic information content (PIC - 0.258), band informativeness (∑Ib - 3.283) and marker index (MI - 0.006) were highest in the case of RsaI enzyme. Our data suggest that the RsaI polymorphic site in the mitochondrial control region is an informative marker for breast cancer development in Mizo population.

KW - Biomarkers, Tumor/genetics

KW - Breast Neoplasms/blood

KW - DNA, Mitochondrial/genetics

KW - Female

KW - Genes, Mitochondrial

KW - Genome/genetics

KW - Genome, Mitochondrial/genetics

KW - Humans

KW - Mitochondria/genetics

KW - Mongolia

KW - Polymerase Chain Reaction

KW - Polymorphism, Genetic

KW - Polymorphism, Restriction Fragment Length/genetics

KW - Sequence Analysis, DNA/methods

KW - Whole Genome Sequencing/methods

U2 - 10.3109/19401736.2014.982627

DO - 10.3109/19401736.2014.982627

M3 - Article

C2 - 25431825

SN - 2470-1394

VL - 27

SP - 2205

EP - 2208

JO - Mitochondrial DNA, Part A: DNA mapping, sequencing, and analysis

JF - Mitochondrial DNA, Part A: DNA mapping, sequencing, and analysis

IS - 3

ER -

Polymorphism in mtDNA control region of Mizo-Mongloid Breast Cancer samples as revealed by PCR-RFLP analysis

Abstract

Subject classification (UKÄ)

Free keywords

UN SDGs

Access to Document

Fingerprint

Cite this