Positively Regulated Glycerol/G3P-Dependent Bacillus subtilis Gene Expression System Based on Anti-Termination.

Anna Lewin, Xiao-Dong Su, Lars Hederstedt

Research output: Contribution to journalArticlepeer-review

2 Citations (SciVal)

Abstract

Plasmid pLALA was constructed for glycerol or glycerol-3-phosphate inducible plasmid-borne gene expression in Bacillus subtilis and closely related Gram-positive bacteria. Gene expression using pLALA is based on anti-termination of transcription and involves the B. subtilis GlpP protein that in the presence of glycerol-3-phosphate acts as an anti-terminator protein by binding to the 5'-untranslated region of glpD mRNA. Properties and the usefulness of the system, denoted LALA, were validated by inducible production in B. subtilis strainsof two water-soluble proteins (beta-galactosidase and a protein phospho-tyrosine phosphatase), and one integral membrane protein (heme A synthase). Advantages with LALA is that it is based on positive control, does not involve a DNA-binding protein, and that glycerol, a cheap and stable compound, can be used as inducer of gene expression.
Original languageEnglish
Pages (from-to)61-70
JournalJournal of Molecular Microbiology and Biotechnology
Volume17
DOIs
Publication statusPublished - 2009

Subject classification (UKÄ)

  • Microbiology

Keywords

  • Anti-termination
  • glp
  • Glycerol inducible
  • Bacillus subtilis

Fingerprint

Dive into the research topics of 'Positively Regulated Glycerol/G3P-Dependent <em>Bacillus subtilis</em> Gene Expression System Based on Anti-Termination.'. Together they form a unique fingerprint.

Cite this