Preparation of a crystallizable mRNA-binding fragment of Moorella thermoacetica elongation factor SelB

Maria Selmer, R Wilting, D Holmlund, Xiao-Dong Su

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3 Citations (SciVal)

Abstract

SelB is a bacterial elongation factor required for the decoding of a UGA stop codon together with a specific mRNA hairpin to selenocysteine. In attempts to crystallize Moorella thermoacetica SelB, a proteolysis process occurred and crystals of a proteolytic fragment were observed. The crystals, which appeared after a year, contained a C-terminal 30 kDa fragment containing the mRNA-binding domain. This fragment was reproduced through recloning. Crystals diffracting to 2.7 Angstrom were obtained.
Original languageEnglish
Pages (from-to)1871-1873
JournalActa Crystallographica. Section D: Biological Crystallography
Volume58
DOIs
Publication statusPublished - 2002

Subject classification (UKÄ)

  • Structural Biology

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