Preparation of cell suspensions for co-transplantation: methodological considerations

A I Othberg, A E Willing, S Saporta, D F Cameron, P R Sanberg

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The purpose of the current study was to determine the optimal strategy for preparing cell suspensions for co-transplantation. In the first experiment, the number of Sertoli cell (SC) aggregates and the number of tyrosine hydroxylase positive neurons were compared over time when cell suspensions of Sertoli or ventral mesencephalic cells were kept as a co-suspension mixed at 0 h. Cells from each suspension were dispensed onto glass slides in a manner similar to transplantation. When dispensed in this manner, the number of SC aggregates and TH-positive neurons decreased over 4 h. In experiment 2, the cell suspensions were mixed just prior to injection at each of four timepoints, the number of aggregates and TH neurons was consistent over time. Clearly this latter strategy resulted consistent recovery of both cell types for transplants up to 3 h after suspension preparation.

Original languageEnglish
Pages (from-to)111-4
Number of pages4
JournalNeuroscience Letters
Volume247
Issue number2-3
DOIs
Publication statusPublished - 1998 May 15
Externally publishedYes

Keywords

  • Animals
  • Biomarkers
  • Brain Tissue Transplantation
  • Cell Aggregation
  • Cell Culture Techniques
  • Cell Survival
  • Cell Transplantation
  • Coculture Techniques
  • Fetal Tissue Transplantation
  • Male
  • Mesencephalon
  • Nerve Tissue Proteins
  • Neurons
  • Rats
  • Rats, Sprague-Dawley
  • Sertoli Cells
  • Suspensions
  • Swine
  • Tyrosine 3-Monooxygenase
  • Journal Article
  • Research Support, Non-U.S. Gov't

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