Presence of a novel form of phosphatidylinositol 4-kinase in rat liver

Henric Olsson, Wilma Martinez Arias, Bjørn K Drøbak, Bengt Jergil

Research output: Contribution to journalArticlepeer-review

Abstract

Rat liver microsomes contain two distinct forms of PtdIns 4-kinase which were resolved by heparin-Sepharose chromatography. One enzyme was identified as the type II PtdIns kinase previously isolated from exocytotic vesicles. The other enzyme, however, was a novel PtdIns 4-kinase isoform with properties differing from any other PtdIns kinase so far characterized. Both kinases were recognized by a monoclonal antibody specific for type II PtdIns 4-kinase, but the novel enzyme was considerably less sensitive to inhibition by adenosine and Ca2+ than type II enzymes, and in addition was specifically inhibited by submillimolar concentrations of dithioerythritol. The presence of a novel PtdIns 4-kinase isoform in rat liver raises the question of whether this enzyme is unique for this organ or whether it has a more widespread distribution but so far has avoided detection.
Original languageEnglish
Pages (from-to)282-286
JournalFEBS Letters
Volume361
Issue number2-3
DOIs
Publication statusPublished - 1995

Subject classification (UKÄ)

  • Biological Sciences

Free keywords

  • Signal transduction
  • Phosphoinositide
  • Phosphatidyl inositol 4-kinase
  • Rat liver

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