Probing Cage Relaxation in Concentrated Protein Solutions by X-Ray Photon Correlation Spectroscopy

Yuriy Chushkin; Alessandro Gulotta; Felix Roosen-Runge; Antara Pal; Anna Stradner; Peter Schurtenberger

doi:10.1103/PhysRevLett.129.238001

Probing Cage Relaxation in Concentrated Protein Solutions by X-Ray Photon Correlation Spectroscopy

Yuriy Chushkin, Alessandro Gulotta, Felix Roosen-Runge, Antara Pal, Anna Stradner, Peter Schurtenberger

Research output: Contribution to journal › Article › peer-review

Abstract

Diffusion of proteins on length scales of their size is crucial for understanding the machinery of living cells. X-ray photon correlation spectroscopy (XPCS) is currently the only way to access long-time collective diffusion on these length scales, but radiation damage so far limits the use in biological systems. We apply a new approach to use XPCS to measure cage relaxation in crowded α-crystallin solutions. This allows us to correct for radiation effects, obtain missing information on long time diffusion, and support the fundamental analogy between protein and colloid dynamical arrest.

Original language	English
Article number	238001
Number of pages	6
Journal	Physical Review Letters
Volume	129
Issue number	23
DOIs	https://doi.org/10.1103/PhysRevLett.129.238001
Publication status	Published - 2022 Dec 2

Subject classification (UKÄ)

Physical Chemistry (including Surface- and Colloid Chemistry)
Biophysics

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abstract = "Diffusion of proteins on length scales of their size is crucial for understanding the machinery of living cells. X-ray photon correlation spectroscopy (XPCS) is currently the only way to access long-time collective diffusion on these length scales, but radiation damage so far limits the use in biological systems. We apply a new approach to use XPCS to measure cage relaxation in crowded α-crystallin solutions. This allows us to correct for radiation effects, obtain missing information on long time diffusion, and support the fundamental analogy between protein and colloid dynamical arrest.",

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AU - Chushkin, Yuriy

AU - Gulotta, Alessandro

AU - Roosen-Runge, Felix

AU - Pal, Antara

AU - Stradner, Anna

AU - Schurtenberger, Peter

PY - 2022/12/2

Y1 - 2022/12/2

N2 - Diffusion of proteins on length scales of their size is crucial for understanding the machinery of living cells. X-ray photon correlation spectroscopy (XPCS) is currently the only way to access long-time collective diffusion on these length scales, but radiation damage so far limits the use in biological systems. We apply a new approach to use XPCS to measure cage relaxation in crowded α-crystallin solutions. This allows us to correct for radiation effects, obtain missing information on long time diffusion, and support the fundamental analogy between protein and colloid dynamical arrest.

AB - Diffusion of proteins on length scales of their size is crucial for understanding the machinery of living cells. X-ray photon correlation spectroscopy (XPCS) is currently the only way to access long-time collective diffusion on these length scales, but radiation damage so far limits the use in biological systems. We apply a new approach to use XPCS to measure cage relaxation in crowded α-crystallin solutions. This allows us to correct for radiation effects, obtain missing information on long time diffusion, and support the fundamental analogy between protein and colloid dynamical arrest.

UR - https://www.scopus.com/pages/publications/85143738867

U2 - 10.1103/PhysRevLett.129.238001

DO - 10.1103/PhysRevLett.129.238001

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JF - Physical Review Letters

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ER -

Probing Cage Relaxation in Concentrated Protein Solutions by X-Ray Photon Correlation Spectroscopy

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Subject classification (UKÄ)

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