Probing control of glucose feeding in Vibrio cholerae cultivations

Lena de Maré, Lena Andersson, Per Hagander

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9 Citations (SciVal)


Infection with Vibrio cholerae is a significant problem in manydeveloping countries. Cultivation of Vibrio cholerae is used inproduction of CTB, which is a component in a cholera vaccine. Fed - batch cultivations with V. cholerae in defined mediahave been conducted and reproducible results were obtained. A probing feeding strategy developed by Åkessonfor E. coli cultivations has been tested.The program is working as well for V. cholerae as forE. coliin minimizing the amount acetic acid formed and avoiding anaerobicconditions. Two hours after the feed start most of the acetic acidaccumulated during the batch phase is consumed. The resulting feedrate tends to be thehighest possible with respect to the constraints from cell metabolismand mass transfer, thus maximizing productivity in terms of biomass.A cell dry weight of 20 - 23 g/l is obtained after12 hours of feeding.
Original languageEnglish
Pages (from-to)221-228
JournalBioprocess and Biosystems Engineering
Issue number4
Publication statusPublished - 2003

Subject classification (UKÄ)

  • Control Engineering


  • fed-batch cultivations
  • V. cholerae
  • probing feeding strategy


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