Protease activity of Blastocystis hominis

Selena W S Sio; Manoj K Puthia; Alex S Y Lee; Jia Lu; Kevin S W Tan

doi:10.1007/s00436-006-0131-1

Protease activity of Blastocystis hominis

Selena W S Sio, Manoj K Puthia, Alex S Y Lee, Jia Lu, Kevin S W Tan

Research output: Contribution to journal › Article › peer-review

Abstract

Parasite-derived proteases are important for the parasite life cycle and the pathogenesis of the disease they produce. Proteases of intestinal protozoan parasite Blastocystis hominis were studied for the first time with azocasein assays and gelatin SDS-PAGE analysis. Parasitic lysates were found to have high protease activity and nine protease bands of low (20-33 kDa) and high (44-75 kDa) molecular weights were reported. Proteases were found to be pH-dependent and highest proteolytic activity was observed at neutral pH. Inhibition studies showed that B. hominis isolate B, like many other protozoan parasites, contains mainly cysteine proteases.

Original language	English
Pages (from-to)	126-30
Journal	Parasitology Research
Volume	99
Issue number	2
DOIs	https://doi.org/10.1007/s00436-006-0131-1
Publication status	Published - 2006 Jul
Externally published	Yes

Subject classification (UKÄ)

Cell and Molecular Biology

Keywords

Animals
Blastocystis hominis/drug effects
Cysteine Endopeptidases/metabolism
Electrophoresis, Polyacrylamide Gel
Humans
Peptide Hydrolases/drug effects
Protease Inhibitors/pharmacology

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10.1007/s00436-006-0131-1

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title = "Protease activity of Blastocystis hominis",

abstract = "Parasite-derived proteases are important for the parasite life cycle and the pathogenesis of the disease they produce. Proteases of intestinal protozoan parasite Blastocystis hominis were studied for the first time with azocasein assays and gelatin SDS-PAGE analysis. Parasitic lysates were found to have high protease activity and nine protease bands of low (20-33 kDa) and high (44-75 kDa) molecular weights were reported. Proteases were found to be pH-dependent and highest proteolytic activity was observed at neutral pH. Inhibition studies showed that B. hominis isolate B, like many other protozoan parasites, contains mainly cysteine proteases.",

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author = "Sio, {Selena W S} and Puthia, {Manoj K} and Lee, {Alex S Y} and Jia Lu and Tan, {Kevin S W}",

year = "2006",

month = jul,

doi = "10.1007/s00436-006-0131-1",

language = "English",

volume = "99",

pages = "126--30",

journal = "Parasitology Research",

issn = "1432-1955",

publisher = "Springer",

number = "2",

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TY - JOUR

T1 - Protease activity of Blastocystis hominis

AU - Sio, Selena W S

AU - Puthia, Manoj K

AU - Lee, Alex S Y

AU - Lu, Jia

AU - Tan, Kevin S W

PY - 2006/7

Y1 - 2006/7

N2 - Parasite-derived proteases are important for the parasite life cycle and the pathogenesis of the disease they produce. Proteases of intestinal protozoan parasite Blastocystis hominis were studied for the first time with azocasein assays and gelatin SDS-PAGE analysis. Parasitic lysates were found to have high protease activity and nine protease bands of low (20-33 kDa) and high (44-75 kDa) molecular weights were reported. Proteases were found to be pH-dependent and highest proteolytic activity was observed at neutral pH. Inhibition studies showed that B. hominis isolate B, like many other protozoan parasites, contains mainly cysteine proteases.

AB - Parasite-derived proteases are important for the parasite life cycle and the pathogenesis of the disease they produce. Proteases of intestinal protozoan parasite Blastocystis hominis were studied for the first time with azocasein assays and gelatin SDS-PAGE analysis. Parasitic lysates were found to have high protease activity and nine protease bands of low (20-33 kDa) and high (44-75 kDa) molecular weights were reported. Proteases were found to be pH-dependent and highest proteolytic activity was observed at neutral pH. Inhibition studies showed that B. hominis isolate B, like many other protozoan parasites, contains mainly cysteine proteases.

KW - Animals

KW - Blastocystis hominis/drug effects

KW - Cysteine Endopeptidases/metabolism

KW - Electrophoresis, Polyacrylamide Gel

KW - Humans

KW - Peptide Hydrolases/drug effects

KW - Protease Inhibitors/pharmacology

U2 - 10.1007/s00436-006-0131-1

DO - 10.1007/s00436-006-0131-1

M3 - Article

C2 - 16518611

VL - 99

SP - 126

EP - 130

JO - Parasitology Research

JF - Parasitology Research

SN - 1432-1955

IS - 2

ER -

Protease activity of Blastocystis hominis

Abstract

Subject classification (UKÄ)

Keywords

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