Protein cold denaturation as seen from the solvent

Monika Davidovic, Carlos Mattea, Johan Qvist, Bertil Halle

Research output: Contribution to journalArticlepeer-review

63 Citations (SciVal)

Abstract

Unlike most ordered molecular systems, globular proteins exhibit a temperature of maximum stability, implying that the structure can be disrupted by cooling. This cold denaturation phenomenon is usually linked to the temperature-dependent hydrophobic driving force for protein folding. Yet, despite the key role played by protein−water interactions, hydration changes during cold denaturation have not been investigated experimentally. Here, we use water−17O spin relaxation to monitor the hydration dynamics of the proteins BPTI, ubiquitin, apomyoglobin, and β-lactoglobulin in aqueous solution from room temperature down to −35 °C. To access this temperature range without ice formation, we contained the protein solution in nonperturbing picoliter emulsion droplets. Among the four proteins, only the destabilized apomyoglobin was observed to cold denature. Ubiquitin was found to be thermodynamically stable at least down to −32 °C, whereas β-lactoglobulin is expected to be unstable below −5 °C but remains kinetically trapped in the native state. When destabilized by 4 M urea, β-lactoglobulin cold denatures at 10 °C, as found previously by other methods. As seen from the solvent, the cold-denatured states of apomyoglobin in water and β-lactoglobulin in 4 M urea are relatively compact and are better described as solvent-penetrated than as unfolded. This finding challenges the popular analogy between cold denaturation and the anomalous low-temperature increase in aqueous solubility of nonpolar molecules. Our results also suggest that the reported cold denaturation at −20 °C of ubiquitin encapsulated in reverse micelles is caused by the low water content rather than by the low temperature.
Original languageEnglish
Pages (from-to)1025-1036
JournalJournal of the American Chemical Society
Volume131
Issue number3
DOIs
Publication statusPublished - 2009

Subject classification (UKÄ)

  • Physical Chemistry

Fingerprint

Dive into the research topics of 'Protein cold denaturation as seen from the solvent'. Together they form a unique fingerprint.

Cite this