Protein D, an immunoglobulin D-binding protein of Haemophilus influenzae: cloning, nucleotide sequence, and expression in Escherichia coli

Håkan Janson, Lars-Olof Hedén, Anders Grubb, Maorong Ruan, Arne Forsgren

Research output: Contribution to journalArticlepeer-review

Abstract

The gene for protein D, a membrane-associated protein with specific affinity for human immunoglobulin D, was cloned from a nontypeable strain of Haemophilus influenzae. The gene was expressed in Escherichia coli from an endogenous promoter, and the gene product has an apparent molecular weight equal to that of H. influenzae protein D (42,000). The complete nucleotide sequence of the gene for protein D was determined, and the deduced amino acid sequence of 364 residues includes a putative signal sequence of 18 amino acids containing a consensus sequence, Leu-Ala-Gly-Cys, for bacterial lipoproteins. The sequence of protein D shows no similarity to those of other immunoglobulin-binding proteins. Protein D is the first example of immunoglobulin receptors from gram-negative bacteria that has been cloned and sequenced.
Original languageEnglish
Pages (from-to)119-125
JournalInfection and Immunity
Volume59
Issue number1
Publication statusPublished - 1991

Subject classification (UKÄ)

  • Pharmacology and Toxicology
  • Microbiology in the medical area
  • Medicinal Chemistry

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