Abstract
Recent years have seen an increasing number of biophysical studies of proteins being conducted in cells and concentrated protein solutions. In these experiments, compared to dilute-solution data, both stabilization and destabilization of globular proteins have been observed, which cannot be explained in terms of volume exclusion alone. For a fundamental understanding of the observed effects, there is a need for computational modeling beyond the level of hard-sphere crowders. This mini-review discusses recent efforts to simulate folding/unfolding properties of proteins in the presence of explicit macromolecular crowders. A Monte Carlo-based approach by us is described, which we recently applied to study the equilibrium folding thermodynamics of two peptides in the presence of explicit protein crowders.
Original language | English |
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Pages (from-to) | 627-638 |
Number of pages | 12 |
Journal | European Physical Journal: Special Topics |
Volume | 226 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2017 Apr 1 |
Subject classification (UKÄ)
- Other Physics Topics
- Biophysics