Protein kinase C inhibition has only a transient growth arresting effect on in vitro regenerating mouse sensory neurons

Peter Wiklund, Per Ekström

Research output: Contribution to journalArticlepeer-review

Abstract

Adult mice sensory ganglia were cultured in an extracellular matrix gel. Analyses of extending axons were made 48 h (long-term) or immediately (short-term) after addition of protein kinase inhibitors. Long- and short-term growth was insensitive to protein kinase A/G inhibition by HA-1004. Long-term protein kinase C inhibition by chelerythrine affected only certain, long axons. In the short-term virtually all axon growth was arrested, but largely recovered on the following day. When combined, the drugs inhibited all long- and short-term growth and largely prevented the recovery of the latter. The transient effect by chelerythrine, and the permanent inhibition after combination with HA-1004, suggests compensatory mechanisms, perhaps via other kinases. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)155-158
Number of pages4
JournalNeuroscience Letters
Volume275
Issue number3
DOIs
Publication statusPublished - 1999 Nov 19

Subject classification (UKÄ)

  • Cell Biology

Free keywords

  • Chelerythrine chloride
  • Dorsal root ganglia
  • Growth cones
  • HA-1004
  • Protein kinase A
  • Protein kinase G

Fingerprint

Dive into the research topics of 'Protein kinase C inhibition has only a transient growth arresting effect on in vitro regenerating mouse sensory neurons'. Together they form a unique fingerprint.

Cite this