Protein-surfactant interactions at solid surfaces

Thomas Arnebrant, Marie C Wahlgren

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Effects of surfactants on protein adsorption are reviewed. Differences between removal of preadsorbed proteins (elutability) and competitive adsorption are discussed and simple models are suggested. It can be concluded that surfactants may interact through solubilization or replacement mechanisms depending on surfactant- surface interactions and surfactant- protein binding. Solubilization requires complex formation between protein and surfactant, and the replacement adsorption of the surfactant to the surface. As for protein adsorption, one of the most important properties affecting the elutability appears to be the conformational stability. Differences between a competitive situation and addition of surfactant after adsorption of the protein are suggested to originate from alteration in surface activity of protein-surfactant complexes formed in solution as compared to pure protein, the difference in diffusivity of surfactants and protein, and time dependent conformational changes of the protein.
Original languageEnglish
Title of host publicationProteins at Interfaces II Fundamentals and Applications
EditorsThomas A. Horbett1 Horbett1, John L. Brash
PublisherThe American Chemical Society (ACS)
Pages239-255
Number of pages17
Volume602
ISBN (Electronic)9780841215276
ISBN (Print)9780841233041
DOIs
Publication statusPublished - 1995

Publication series

NameACS Symposium Series
PublisherAmerican Chemical Society
Volume602

Subject classification (UKÄ)

  • Physical Chemistry

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