Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients

Pia Davidsson; Ann Westman-Brinkmalm; Carol L Nilsson; Maria Lindbjer; Linda Paulson; Niels Andreasen; Magnus Sjögren; Kaj Blennow

Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients

Pia Davidsson, Ann Westman-Brinkmalm, Carol L Nilsson, Maria Lindbjer, Linda Paulson, Niels Andreasen, Magnus Sjögren, Kaj Blennow

University of Gothenburg

Research output: Contribution to journal › Article › peer-review

Abstract

By comparing the CSF proteome between Alzheimer disease (AD) patients and controls it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. We used mini-gel technology in a two-dimensional electrophoresis procedure, sensitive SYPRO Ruby staining and mass spectrometry for clinical screening of disease-influenced CSF proteins in 15 AD patients and 12 controls. The levels of six proteins and their isoforms, including proapolipoprotein, apolipoprotein E, beta-2 microglobulin, retinol-binding protein, transthyretin, and ubiquitin, were significantly altered in CSF of AD patients. The most prominently altered proteins were the apolipoproteins, especially proapolipoprotein.

Original language	English
Pages (from-to)	611-5
Number of pages	5
Journal	NeuroReport
Volume	13
Issue number	5
Publication status	Published - 2002 Apr 16
Externally published	Yes

Free keywords

Aged
Aged, 80 and over
Alzheimer Disease
Confidence Intervals
Female
Humans
Longitudinal Studies
Male
Patients
Protein Isoforms
Proteins
Proteome
Statistics, Nonparametric
Comparative Study

Cite this

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title = "Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients",

abstract = "By comparing the CSF proteome between Alzheimer disease (AD) patients and controls it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. We used mini-gel technology in a two-dimensional electrophoresis procedure, sensitive SYPRO Ruby staining and mass spectrometry for clinical screening of disease-influenced CSF proteins in 15 AD patients and 12 controls. The levels of six proteins and their isoforms, including proapolipoprotein, apolipoprotein E, beta-2 microglobulin, retinol-binding protein, transthyretin, and ubiquitin, were significantly altered in CSF of AD patients. The most prominently altered proteins were the apolipoproteins, especially proapolipoprotein.",

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TY - JOUR

T1 - Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients

AU - Davidsson, Pia

AU - Westman-Brinkmalm, Ann

AU - Nilsson, Carol L

AU - Lindbjer, Maria

AU - Paulson, Linda

AU - Andreasen, Niels

AU - Sjögren, Magnus

AU - Blennow, Kaj

PY - 2002/4/16

Y1 - 2002/4/16

N2 - By comparing the CSF proteome between Alzheimer disease (AD) patients and controls it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. We used mini-gel technology in a two-dimensional electrophoresis procedure, sensitive SYPRO Ruby staining and mass spectrometry for clinical screening of disease-influenced CSF proteins in 15 AD patients and 12 controls. The levels of six proteins and their isoforms, including proapolipoprotein, apolipoprotein E, beta-2 microglobulin, retinol-binding protein, transthyretin, and ubiquitin, were significantly altered in CSF of AD patients. The most prominently altered proteins were the apolipoproteins, especially proapolipoprotein.

AB - By comparing the CSF proteome between Alzheimer disease (AD) patients and controls it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. We used mini-gel technology in a two-dimensional electrophoresis procedure, sensitive SYPRO Ruby staining and mass spectrometry for clinical screening of disease-influenced CSF proteins in 15 AD patients and 12 controls. The levels of six proteins and their isoforms, including proapolipoprotein, apolipoprotein E, beta-2 microglobulin, retinol-binding protein, transthyretin, and ubiquitin, were significantly altered in CSF of AD patients. The most prominently altered proteins were the apolipoproteins, especially proapolipoprotein.

KW - Aged

KW - Aged, 80 and over

KW - Alzheimer Disease

KW - Confidence Intervals

KW - Female

KW - Humans

KW - Longitudinal Studies

KW - Male

KW - Patients

KW - Protein Isoforms

KW - Proteins

KW - Proteome

KW - Statistics, Nonparametric

KW - Comparative Study

M3 - Article

C2 - 11973456

SN - 0959-4965

VL - 13

SP - 611

EP - 615

JO - NeuroReport

JF - NeuroReport

IS - 5

ER -

Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients

Abstract

Free keywords

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