Proteomic Profiling of Diffuse Large B-Cell Lymphomas

Anna Kwiecińska, Anna Porwit, Nazariy Souchelnytskyi, Ann Kaufeldt, Catharina Larsson, Svetlana Bajalica-Lagercrantz, Serhiy Souchelnytskyi

Research output: Contribution to journalArticlepeer-review

3 Citations (SciVal)

Abstract

Objective: The aim of this study was to identify differences in proteome profiles of diffuse large B-cell lymphoma (DLBCL) of nongerminal center (non-GC) versus GC type in the search for new markers and drug targets. Methods: Six DLBCL, with 3 repeats for each, were used for the initial study by proteomics: 3 non-GC and 3 GC DLBCL cases. For immunohistochemistry, tissue microarrays were made from 31 DLBCL samples: 16 non-GC de novo lymphomas and 15 GC cases (11 transformed from follicular lymphomas and 4 de novo GC lymphomas). Proteome profiling was performed by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. Results: Ninety-one proteins were found differentially expressed in non-GC compared to GC type. The Cytoscape tool was used for systemic analysis of proteomics data, revealing 19 subnetworks representing functions affected in non-GC versus GC types of DLBCL. Conclusion: A validation study of 3 selected proteins (BiP/Grp78, Hsp90, and cyclin B2) showed the enhanced expression in non-GC DLBCL, supporting the proteomics data.

Original languageEnglish
Pages (from-to)211-219
JournalPathobiology
Volume85
Issue number4
Early online date2018 Apr 4
DOIs
Publication statusPublished - 2018

Subject classification (UKÄ)

  • Cancer and Oncology

Keywords

  • BiP/Grp78
  • Cyclin B2
  • De novo diffuse large B-cell lymphomas
  • Diffuse large B-cell lymphoma
  • Germinal center type
  • Hsp90
  • Non-germinal center type
  • Proteomics
  • Transformed lymphoma

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