Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

Jessica Giacomoni; Mette Habekost; Efrain Cepeda-Prado; Bengt Mattsson; Daniella Rylander Ottosson; Malin Parmar; Janko Kajtez

doi:10.1016/j.xpro.2022.102041

Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

Jessica Giacomoni, Mette Habekost, Efrain Cepeda-Prado, Bengt Mattsson, Daniella Rylander Ottosson , Malin Parmar, Janko Kajtez

University of Copenhagen

Research output: Contribution to journal › Article › peer-review

Abstract

Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.

Original language	English
Article number	102041
Journal	STAR Protocols
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2022.102041
Publication status	Published - 2023

Subject classification (UKÄ)

Other Medical Engineering

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10.1016/j.xpro.2022.102041Licence: CC BY

Cite this

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title = "Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices",

abstract = "Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.",

author = "Jessica Giacomoni and Mette Habekost and Efrain Cepeda-Prado and Bengt Mattsson and \{Rylander Ottosson\}, Daniella and Malin Parmar and Janko Kajtez",

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AU - Giacomoni, Jessica

AU - Habekost, Mette

AU - Cepeda-Prado, Efrain

AU - Mattsson, Bengt

AU - Rylander Ottosson , Daniella

AU - Parmar, Malin

AU - Kajtez, Janko

PY - 2023

Y1 - 2023

N2 - Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.

AB - Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.

UR - https://www.scopus.com/pages/publications/85174640568

U2 - 10.1016/j.xpro.2022.102041

DO - 10.1016/j.xpro.2022.102041

M3 - Article

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JO - STAR Protocols

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ER -

Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

Abstract

Subject classification (UKÄ)

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NSC-Reconstruct: Novel Strategies for Cell-based Neural Reconstruction

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Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

Abstract

Subject classification (UKÄ)

Access to Document

Other files and links

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NSC-Reconstruct: Novel Strategies for Cell-based Neural Reconstruction

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