Pulsed electric field reduces the permeability of potato cell wall

Federico Gomez; P Thomas Vernier; Petr Dejmek; Antonio Vicente; Martin A Gundersen

doi:10.1002/bem.20394

Pulsed electric field reduces the permeability of potato cell wall

Federico Gomez, P Thomas Vernier, Petr Dejmek, Antonio Vicente, Martin A Gundersen

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FMI-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.

Original language	English
Pages (from-to)	296-301
Journal	Bioelectromagnetics
Volume	29
Issue number	4
DOIs	https://doi.org/10.1002/bem.20394
Publication status	Published - 2008

Subject classification (UKÄ)

Food Engineering

Free keywords

oxidative cross-linking
stress
electric fields
cell wall
FM1-43

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10.1002/bem.20394

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title = "Pulsed electric field reduces the permeability of potato cell wall",

abstract = "The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FMI-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.",

keywords = "oxidative cross-linking, stress, electric fields, cell wall, FM1-43",

author = "Federico Gomez and Vernier, {P Thomas} and Petr Dejmek and Antonio Vicente and Gundersen, {Martin A}",

year = "2008",

doi = "10.1002/bem.20394",

language = "English",

volume = "29",

pages = "296--301",

journal = "Bioelectromagnetics",

issn = "0197-8462",

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TY - JOUR

T1 - Pulsed electric field reduces the permeability of potato cell wall

AU - Gomez, Federico

AU - Vernier, P Thomas

AU - Dejmek, Petr

AU - Vicente, Antonio

AU - Gundersen, Martin A

PY - 2008

Y1 - 2008

N2 - The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FMI-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.

AB - The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FMI-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.

KW - oxidative cross-linking

KW - stress

KW - electric fields

KW - cell wall

KW - FM1-43

U2 - 10.1002/bem.20394

DO - 10.1002/bem.20394

M3 - Article

C2 - 18163439

SN - 0197-8462

VL - 29

SP - 296

EP - 301

JO - Bioelectromagnetics

JF - Bioelectromagnetics

IS - 4

ER -

Pulsed electric field reduces the permeability of potato cell wall

Abstract

Subject classification (UKÄ)

Free keywords

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