Purification and Functional Characterization of C4b-Binding Protein (C4BP).

Frida Mohlin; Anna Blom

doi:10.1007/978-1-62703-724-2_14

Purification and Functional Characterization of C4b-Binding Protein (C4BP).

Frida Mohlin, Anna Blom

Protein Chemistry, Malmö

Research output: Contribution to journal › Article › peer-review

Abstract

C4b-binding protein (C4BP) is a soluble, 570 kDa large glycoprotein, present in plasma at a concentration of approximately 200 mg/L. C4BP is the main inhibitor of the classical and lectin pathways of complement, where it controls C4b-mediated reactions. Here, we describe a method for purification of C4BP from human plasma, which is based on barium chloride precipitation, anion exchange chromatography, and gel filtration. We also describe a functional assay, in which C4BP's cofactor activity to factor I, in the degradation of C4b, can be assessed.

Original language	English
Pages (from-to)	169-176
Journal	Methods in Molecular Biology
Volume	1100
DOIs	https://doi.org/10.1007/978-1-62703-724-2_14
Publication status	Published - 2014

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10.1007/978-1-62703-724-2_14

http://www.ncbi.nlm.nih.gov/pubmed/24218259?dopt=Abstract

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title = "Purification and Functional Characterization of C4b-Binding Protein (C4BP).",

abstract = "C4b-binding protein (C4BP) is a soluble, 570 kDa large glycoprotein, present in plasma at a concentration of approximately 200 mg/L. C4BP is the main inhibitor of the classical and lectin pathways of complement, where it controls C4b-mediated reactions. Here, we describe a method for purification of C4BP from human plasma, which is based on barium chloride precipitation, anion exchange chromatography, and gel filtration. We also describe a functional assay, in which C4BP's cofactor activity to factor I, in the degradation of C4b, can be assessed.",

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AB - C4b-binding protein (C4BP) is a soluble, 570 kDa large glycoprotein, present in plasma at a concentration of approximately 200 mg/L. C4BP is the main inhibitor of the classical and lectin pathways of complement, where it controls C4b-mediated reactions. Here, we describe a method for purification of C4BP from human plasma, which is based on barium chloride precipitation, anion exchange chromatography, and gel filtration. We also describe a functional assay, in which C4BP's cofactor activity to factor I, in the degradation of C4b, can be assessed.

UR - https://www.scopus.com/pages/publications/84934444808

U2 - 10.1007/978-1-62703-724-2_14

DO - 10.1007/978-1-62703-724-2_14

M3 - Article

SN - 1940-6029

VL - 1100

SP - 169

EP - 176

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Purification and Functional Characterization of C4b-Binding Protein (C4BP).

Abstract

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