Purification and functional characterization of factor I.

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1 Citation (SciVal)

Abstract

Factor I (FI) is a soluble, 88 kDa glycoprotein present in plasma at a concentration of approximately 35 mg/L. FI inhibits all complement pathways as it degrades activated C4b and C3b when these are bound to a cofactor such as C4b-binding protein or factor H. Here, we describe a method for purification of FI from human plasma, which is based on affinity chromatography followed by anion exchange chromatography. We also describe a functional assay, in which activity of FI can be assessed.
Original languageEnglish
Pages (from-to)177-188
JournalMethods in Molecular Biology
Volume1100
DOIs
Publication statusPublished - 2014

Subject classification (UKÄ)

  • Other Basic Medicine

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