Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

Huy Cuong Tran; Olivier Van Aken

doi:10.1007/978-1-0716-1653-6_1

Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Abstract

The study of plant mitochondria often requires isolation of mitochondria from plant tissues in intact and functional form. Here, we describe an effective procedure of mitochondrial isolation from leaf tissues and whole seedlings of the model dicot species Arabidopsis thaliana by using differential centrifugation and continuous Percoll density gradients.

Original language	English
Title of host publication	Plant Mitochondria
Subtitle of host publication	Methods and Protocols
Editors	Olivier Van Aken, Allan G. Rasmusson
Publisher	Humana Press
Pages	1-12
Number of pages	12
ISBN (Electronic)	978-1-0716-1653-6
ISBN (Print)	978-1-0716-1652-9
DOIs	https://doi.org/10.1007/978-1-0716-1653-6_1
Publication status	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2363
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Subject classification (UKÄ)

Biochemistry and Molecular Biology
Cell Biology

Keywords

Arabidopsis
Density gradients
Mitochondria
Mitochondrial isolation

Access to Document

10.1007/978-1-0716-1653-6_1

Cite this

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abstract = "The study of plant mitochondria often requires isolation of mitochondria from plant tissues in intact and functional form. Here, we describe an effective procedure of mitochondrial isolation from leaf tissues and whole seedlings of the model dicot species Arabidopsis thaliana by using differential centrifugation and continuous Percoll density gradients.",

keywords = "Arabidopsis, Density gradients, Mitochondria, Mitochondrial isolation",

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Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients. / Tran, Huy Cuong ; Van Aken, Olivier.

Plant Mitochondria: Methods and Protocols. ed. / Olivier Van Aken; Allan G. Rasmusson. Humana Press, 2022. p. 1-12 (Methods in Molecular Biology; Vol. 2363).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

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Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

Abstract

Publication series

Subject classification (UKÄ)

Keywords

Access to Document

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Characterization of land plant-specific proteins required for mitochondrial translation initiation

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