Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation

Sarote Nitsawang, Rajni Hatti-Kaul, Pawinee Kanasawuda

Research output: Contribution to journalArticlepeer-review

96 Citations (SciVal)

Abstract

Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dependent on the initial protein concentration in the material used for processing. Highly pure papain was obtained in a much shorter processing time directly from unclarified latex with the use of an aqueous two-phase system consisting of 8% (w/w) polyethylene glycol and 15% (w/w) ammonium sulfate. (
Original languageEnglish
Pages (from-to)1103-1107
JournalEnzyme and Microbial Technology
Volume39
Issue number5
DOIs
Publication statusPublished - 2006

Subject classification (UKÄ)

  • Industrial Biotechnology

Keywords

  • salt precipitation
  • aqueous two-phase extraction
  • Carica papaya latex
  • papain
  • two-step

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