PXL01 alters macrophage response with no effect on axonal outgrowth or Schwann cell response after nerve repair in rats

Derya Burcu Hazer Rosberg; Lena Stenberg; Margit Mahlapuu; Lars B Dahlin

doi:10.1080/17460751.2024.2361515

PXL01 alters macrophage response with no effect on axonal outgrowth or Schwann cell response after nerve repair in rats

Derya Burcu Hazer Rosberg, Lena Stenberg, Margit Mahlapuu, Lars B Dahlin

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Adjunctive pharmacological treatment may improve nerve regeneration. We investigated nerve regeneration processes of PXL01 - a lactoferrin-derived peptide - after repair of the sciatic nerve in healthy Wistar rats. Materials & methods: PXL01, sodium hyaluronate (carrier) or sodium chloride was administered around the repair. After 6 days axonal outgrowth, Schwann cell response, pan- (CD68) and pro-healing (CD206) macrophages in sciatic nerve, sensory neuronal response in dorsal root ganglia (DRG) and expression of heat shock protein 27 (HSP27) in sciatic nerves and DRGs were analyzed. Results: Despite a lower number of pan-macrophages, other investigated variables in sciatic nerves or DRGs did not differ between the treatment groups. Conclusion: PLX01 applied locally inhibits inflammation through pan-macrophages in repaired sciatic nerves without any impact on nerve regeneration or pro-healing macrophages.

Original language	English
Pages (from-to)	327-343
Journal	Regenerative Medicine
Volume	19
Issue number	6
DOIs	https://doi.org/10.1080/17460751.2024.2361515
Publication status	Published - 2024 Jun 2

Subject classification (UKÄ)

Neurology

Free keywords

Animals
Schwann Cells/metabolism
Macrophages/metabolism
Nerve Regeneration/drug effects
Rats, Wistar
Sciatic Nerve/drug effects
Axons/drug effects
Rats
Ganglia, Spinal/drug effects
Male

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1080/17460751.2024.2361515

1 Doctoral Thesis (compilation)

Nerve regeneration in healthy and diabetic rat sciatic nerves: Influence of timing, a novel scaffold and the lactoferrin-derived peptide PXL01
Hazer, D. B., 2024, Lund: Lund University, Faculty of Medicine. 126 p.
Research output: Thesis › Doctoral Thesis (compilation)

File

Cite this

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title = "PXL01 alters macrophage response with no effect on axonal outgrowth or Schwann cell response after nerve repair in rats",

abstract = " Background: Adjunctive pharmacological treatment may improve nerve regeneration. We investigated nerve regeneration processes of PXL01 - a lactoferrin-derived peptide - after repair of the sciatic nerve in healthy Wistar rats. Materials & methods: PXL01, sodium hyaluronate (carrier) or sodium chloride was administered around the repair. After 6 days axonal outgrowth, Schwann cell response, pan- (CD68) and pro-healing (CD206) macrophages in sciatic nerve, sensory neuronal response in dorsal root ganglia (DRG) and expression of heat shock protein 27 (HSP27) in sciatic nerves and DRGs were analyzed. Results: Despite a lower number of pan-macrophages, other investigated variables in sciatic nerves or DRGs did not differ between the treatment groups. Conclusion: PLX01 applied locally inhibits inflammation through pan-macrophages in repaired sciatic nerves without any impact on nerve regeneration or pro-healing macrophages. ",

keywords = "Animals, Schwann Cells/metabolism, Macrophages/metabolism, Nerve Regeneration/drug effects, Rats, Wistar, Sciatic Nerve/drug effects, Axons/drug effects, Rats, Ganglia, Spinal/drug effects, Male",

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year = "2024",

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day = "2",

doi = "10.1080/17460751.2024.2361515",

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journal = "Regenerative Medicine",

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TY - JOUR

T1 - PXL01 alters macrophage response with no effect on axonal outgrowth or Schwann cell response after nerve repair in rats

AU - Hazer Rosberg, Derya Burcu

AU - Stenberg, Lena

AU - Mahlapuu, Margit

AU - Dahlin, Lars B

PY - 2024/6/2

Y1 - 2024/6/2

N2 - Background: Adjunctive pharmacological treatment may improve nerve regeneration. We investigated nerve regeneration processes of PXL01 - a lactoferrin-derived peptide - after repair of the sciatic nerve in healthy Wistar rats. Materials & methods: PXL01, sodium hyaluronate (carrier) or sodium chloride was administered around the repair. After 6 days axonal outgrowth, Schwann cell response, pan- (CD68) and pro-healing (CD206) macrophages in sciatic nerve, sensory neuronal response in dorsal root ganglia (DRG) and expression of heat shock protein 27 (HSP27) in sciatic nerves and DRGs were analyzed. Results: Despite a lower number of pan-macrophages, other investigated variables in sciatic nerves or DRGs did not differ between the treatment groups. Conclusion: PLX01 applied locally inhibits inflammation through pan-macrophages in repaired sciatic nerves without any impact on nerve regeneration or pro-healing macrophages.

AB - Background: Adjunctive pharmacological treatment may improve nerve regeneration. We investigated nerve regeneration processes of PXL01 - a lactoferrin-derived peptide - after repair of the sciatic nerve in healthy Wistar rats. Materials & methods: PXL01, sodium hyaluronate (carrier) or sodium chloride was administered around the repair. After 6 days axonal outgrowth, Schwann cell response, pan- (CD68) and pro-healing (CD206) macrophages in sciatic nerve, sensory neuronal response in dorsal root ganglia (DRG) and expression of heat shock protein 27 (HSP27) in sciatic nerves and DRGs were analyzed. Results: Despite a lower number of pan-macrophages, other investigated variables in sciatic nerves or DRGs did not differ between the treatment groups. Conclusion: PLX01 applied locally inhibits inflammation through pan-macrophages in repaired sciatic nerves without any impact on nerve regeneration or pro-healing macrophages.

KW - Animals

KW - Schwann Cells/metabolism

KW - Macrophages/metabolism

KW - Nerve Regeneration/drug effects

KW - Rats, Wistar

KW - Sciatic Nerve/drug effects

KW - Axons/drug effects

KW - Rats

KW - Ganglia, Spinal/drug effects

KW - Male

U2 - 10.1080/17460751.2024.2361515

DO - 10.1080/17460751.2024.2361515

M3 - Article

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SN - 1746-0751

VL - 19

SP - 327

EP - 343

JO - Regenerative Medicine

JF - Regenerative Medicine

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ER -