Quantitative analysis of islet glutamic acid decarboxylase p64 autoantibodies in insulin-dependent diabetes mellitus

Heike Bärmeier; Jarl Ahlmén; Mono Landin-Olsson; Ray V. Rajotte; Göran Sundkvist; Garth Warnock; Åke Lernmark

doi:10.3109/08916939209004823

Quantitative analysis of islet glutamic acid decarboxylase p64 autoantibodies in insulin-dependent diabetes mellitus

Heike Bärmeier, Jarl Ahlmén, Mono Landin-Olsson, Ray V. Rajotte, Göran Sundkvist, Garth Warnock, Åke Lernmark

Research output: Contribution to journal › Article › peer-review

Abstract

Autoantibodies against the βcell Mr 64,000 protein (p64), recently identified as an isoform of glutamic acid decarboxylase (GAD), are prevalent in patients with insulin-dependent diabetes mellitus (IDDM). Dog islets were found to represent an abundant source of native p64 allowing the study of antigen-antibody interactions in IDDM. A quantitative, standardized assay for p64 antibodies based on dog islets was developed and evaluated. Utilizing dog and human islets the p64 antibodies were detected in 17/19 (89%) new onset 15-32-year-old patients, compared to 15/19 (79%) in a rat islet assay. ICA were detected in 15/19 (79%) patients and correlated with the presence of p64 antibodies (r_s=0.59, P < 0.004) but not with age at onset, sex, or C-peptide levels. Sensitivity therefore is improved with the dog islet p64 antibody assay which will allow future studies requiring native p64 antigen in larger quantities are possible based on our findings.

Original language	English
Pages (from-to)	187-196
Journal	Autoimmunity
Volume	13
Issue number	3
DOIs	https://doi.org/10.3109/08916939209004823
Publication status	Published - 1992 Jan 1
Externally published	Yes

Subject classification (UKÄ)

Endocrinology and Diabetes

Free keywords

Autoantibodies
Autoantigen
Dog islets
Immunoprecipitation
Islet cell antibodies

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3109/08916939209004823

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title = "Quantitative analysis of islet glutamic acid decarboxylase p64 autoantibodies in insulin-dependent diabetes mellitus",

abstract = "Autoantibodies against the βcell Mr 64,000 protein (p64), recently identified as an isoform of glutamic acid decarboxylase (GAD), are prevalent in patients with insulin-dependent diabetes mellitus (IDDM). Dog islets were found to represent an abundant source of native p64 allowing the study of antigen-antibody interactions in IDDM. A quantitative, standardized assay for p64 antibodies based on dog islets was developed and evaluated. Utilizing dog and human islets the p64 antibodies were detected in 17/19 (89%) new onset 15-32-year-old patients, compared to 15/19 (79%) in a rat islet assay. ICA were detected in 15/19 (79%) patients and correlated with the presence of p64 antibodies (rs=0.59, P < 0.004) but not with age at onset, sex, or C-peptide levels. Sensitivity therefore is improved with the dog islet p64 antibody assay which will allow future studies requiring native p64 antigen in larger quantities are possible based on our findings.",

keywords = "Autoantibodies, Autoantigen, Dog islets, Immunoprecipitation, Islet cell antibodies",

author = "Heike B{\"a}rmeier and Jarl Ahlm{\'e}n and Mono Landin-Olsson and Rajotte, {Ray V.} and G{\"o}ran Sundkvist and Garth Warnock and {\AA}ke Lernmark",

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doi = "10.3109/08916939209004823",

language = "English",

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T1 - Quantitative analysis of islet glutamic acid decarboxylase p64 autoantibodies in insulin-dependent diabetes mellitus

AU - Bärmeier, Heike

AU - Ahlmén, Jarl

AU - Landin-Olsson, Mono

AU - Rajotte, Ray V.

AU - Sundkvist, Göran

AU - Warnock, Garth

AU - Lernmark, Åke

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Autoantibodies against the βcell Mr 64,000 protein (p64), recently identified as an isoform of glutamic acid decarboxylase (GAD), are prevalent in patients with insulin-dependent diabetes mellitus (IDDM). Dog islets were found to represent an abundant source of native p64 allowing the study of antigen-antibody interactions in IDDM. A quantitative, standardized assay for p64 antibodies based on dog islets was developed and evaluated. Utilizing dog and human islets the p64 antibodies were detected in 17/19 (89%) new onset 15-32-year-old patients, compared to 15/19 (79%) in a rat islet assay. ICA were detected in 15/19 (79%) patients and correlated with the presence of p64 antibodies (rs=0.59, P < 0.004) but not with age at onset, sex, or C-peptide levels. Sensitivity therefore is improved with the dog islet p64 antibody assay which will allow future studies requiring native p64 antigen in larger quantities are possible based on our findings.

AB - Autoantibodies against the βcell Mr 64,000 protein (p64), recently identified as an isoform of glutamic acid decarboxylase (GAD), are prevalent in patients with insulin-dependent diabetes mellitus (IDDM). Dog islets were found to represent an abundant source of native p64 allowing the study of antigen-antibody interactions in IDDM. A quantitative, standardized assay for p64 antibodies based on dog islets was developed and evaluated. Utilizing dog and human islets the p64 antibodies were detected in 17/19 (89%) new onset 15-32-year-old patients, compared to 15/19 (79%) in a rat islet assay. ICA were detected in 15/19 (79%) patients and correlated with the presence of p64 antibodies (rs=0.59, P < 0.004) but not with age at onset, sex, or C-peptide levels. Sensitivity therefore is improved with the dog islet p64 antibody assay which will allow future studies requiring native p64 antigen in larger quantities are possible based on our findings.

KW - Autoantibodies

KW - Autoantigen

KW - Dog islets

KW - Immunoprecipitation

KW - Islet cell antibodies

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VL - 13

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JO - Autoimmunity

JF - Autoimmunity

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Quantitative analysis of islet glutamic acid decarboxylase p64 autoantibodies in insulin-dependent diabetes mellitus

Abstract

Subject classification (UKÄ)

Free keywords

UN SDGs

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