Quantitative analysis of rat Ig (sub) classes binding to cell surface antigens

Rune Nilsson; Thomas Brodin; Hans Olov Sjögren

doi:10.1016/0022-1759(82)90030-8

Quantitative analysis of rat Ig (sub) classes binding to cell surface antigens

Rune Nilsson, Thomas Brodin, Hans Olov Sjögren

Research output: Contribution to journal › Article › peer-review

Abstract

An indirect 125I-labeled protein A assay for detection of cell surface-bound rat immunoglobulins is presented. The assay is quantitative and rapid and detects as little as 1 ng of cell surface-bound Ig. It discriminates between antibodies belonging to different IgG subclasses, IgM and IgA. We describe the production and specificity control of the reagents used and show that the test can be used for quantitative analysis. A large number of sera from untreated rats are tested to evaluate the frequency of falsely positive responses and variation due to age, sex and strain of rat. With this test it is relatively easy to quantitate the binding of classes and subclasses of rat immunoglobulins in a small volume (6 μl) of untreated serum.

Original language	English
Pages (from-to)	179-191
Journal	Journal of Immunological Methods
Volume	55
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(82)90030-8
Publication status	Published - 1982

Subject classification (UKÄ)

Immunology in the medical area

Free keywords

protein A
cell surface antigens
rat Ig (sub)classes
binding test
monoclonal Ig

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10.1016/0022-1759(82)90030-8

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title = "Quantitative analysis of rat Ig (sub) classes binding to cell surface antigens",

abstract = "An indirect 125I-labeled protein A assay for detection of cell surface-bound rat immunoglobulins is presented. The assay is quantitative and rapid and detects as little as 1 ng of cell surface-bound Ig. It discriminates between antibodies belonging to different IgG subclasses, IgM and IgA. We describe the production and specificity control of the reagents used and show that the test can be used for quantitative analysis. A large number of sera from untreated rats are tested to evaluate the frequency of falsely positive responses and variation due to age, sex and strain of rat. With this test it is relatively easy to quantitate the binding of classes and subclasses of rat immunoglobulins in a small volume (6 μl) of untreated serum.",

keywords = "protein A, cell surface antigens, rat Ig (sub)classes, binding test, monoclonal Ig",

author = "Rune Nilsson and Thomas Brodin and Sj{\"o}gren, {Hans Olov}",

year = "1982",

doi = "10.1016/0022-1759(82)90030-8",

language = "English",

volume = "55",

pages = "179--191",

journal = "Journal of Immunological Methods",

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T1 - Quantitative analysis of rat Ig (sub) classes binding to cell surface antigens

AU - Nilsson, Rune

AU - Brodin, Thomas

AU - Sjögren, Hans Olov

PY - 1982

Y1 - 1982

N2 - An indirect 125I-labeled protein A assay for detection of cell surface-bound rat immunoglobulins is presented. The assay is quantitative and rapid and detects as little as 1 ng of cell surface-bound Ig. It discriminates between antibodies belonging to different IgG subclasses, IgM and IgA. We describe the production and specificity control of the reagents used and show that the test can be used for quantitative analysis. A large number of sera from untreated rats are tested to evaluate the frequency of falsely positive responses and variation due to age, sex and strain of rat. With this test it is relatively easy to quantitate the binding of classes and subclasses of rat immunoglobulins in a small volume (6 μl) of untreated serum.

AB - An indirect 125I-labeled protein A assay for detection of cell surface-bound rat immunoglobulins is presented. The assay is quantitative and rapid and detects as little as 1 ng of cell surface-bound Ig. It discriminates between antibodies belonging to different IgG subclasses, IgM and IgA. We describe the production and specificity control of the reagents used and show that the test can be used for quantitative analysis. A large number of sera from untreated rats are tested to evaluate the frequency of falsely positive responses and variation due to age, sex and strain of rat. With this test it is relatively easy to quantitate the binding of classes and subclasses of rat immunoglobulins in a small volume (6 μl) of untreated serum.

KW - protein A

KW - cell surface antigens

KW - rat Ig (sub)classes

KW - binding test

KW - monoclonal Ig

U2 - 10.1016/0022-1759(82)90030-8

DO - 10.1016/0022-1759(82)90030-8

M3 - Article

SN - 1872-7905

VL - 55

SP - 179

EP - 191

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 2

ER -

Quantitative analysis of rat Ig (sub) classes binding to cell surface antigens

Abstract

Subject classification (UKÄ)

Free keywords

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