Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells

Jimmy Rodriguez Murillo; Livia Goto-Silva; Aniel Sánchez; Fábio C.S. Nogueira; Gilberto B. Domont; Magno Junqueira

doi:10.1016/j.euprot.2017.06.001

Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells

Jimmy Rodriguez Murillo, Livia Goto-Silva, Aniel Sánchez, Fábio C.S. Nogueira, Gilberto B. Domont, Magno Junqueira

Clinical Protein Science and Imaging

Research output: Contribution to journal › Article › peer-review

Abstract

SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation.

Original language	English
Pages (from-to)	1-11
Number of pages	11
Journal	EuPA Open Proteomics
Volume	16
DOIs	https://doi.org/10.1016/j.euprot.2017.06.001
Publication status	Published - 2017 Sept 1

Subject classification (UKÄ)

Cell and Molecular Biology
Neurosciences

Free keywords

iTRAQ-based proteomics
Neuronal differentiation
Phosphoproteomics
SH-SY5Y cells

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10.1016/j.euprot.2017.06.001

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title = "Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells",

abstract = "SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation.",

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author = "Murillo, {Jimmy Rodriguez} and Livia Goto-Silva and Aniel S{\'a}nchez and Nogueira, {F{\'a}bio C.S.} and Domont, {Gilberto B.} and Magno Junqueira",

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doi = "10.1016/j.euprot.2017.06.001",

language = "English",

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T1 - Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells

AU - Murillo, Jimmy Rodriguez

AU - Goto-Silva, Livia

AU - Sánchez, Aniel

AU - Nogueira, Fábio C.S.

AU - Domont, Gilberto B.

AU - Junqueira, Magno

PY - 2017/9/1

Y1 - 2017/9/1

N2 - SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation.

AB - SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation.

KW - iTRAQ-based proteomics

KW - Neuronal differentiation

KW - Phosphoproteomics

KW - SH-SY5Y cells

U2 - 10.1016/j.euprot.2017.06.001

DO - 10.1016/j.euprot.2017.06.001

M3 - Article

C2 - 29900121

AN - SCOPUS:85023609384

SN - 2212-9685

VL - 16

SP - 1

EP - 11

JO - EuPA Open Proteomics

JF - EuPA Open Proteomics

ER -

Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells

Abstract

Subject classification (UKÄ)

Free keywords

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