Radio-iodinated and internally labelled (35S) IgM monoclonal antibodies in a syngenic rat model

Q Wanying; T Brodin; Crister Ceberg; Christian Ingvar; Kristina Norrgren; H O Sjogren; Sven-Erik Strand

doi:10.3109/02841869109092390

Radio-iodinated and internally labelled (35S) IgM monoclonal antibodies in a syngenic rat model

Q Wanying, T Brodin, Crister Ceberg, Christian Ingvar, Kristina Norrgren, H O Sjogren, Sven-Erik Strand

Research output: Contribution to journal › Article › peer-review

Abstract

To simulate the human situation concerning human monoclonal antibodies (MAbs), we have introduced a new syngenic rat model with an implanted rat colon carcinoma. Rat IgM MAbs (10B12), labelled by the chloramine-T method with 125I or internally with 35S, were injected intravenously into the rats and the biodistribution was studied for 8 days. The radioactivity uptake in the tumours of the 35S label was higher than that of the 125I label and the retention of 35S in the tumours gave tumour/blood ratios 8 times higher than those of 125I at 48 and 96 h after injection. In this model we have shown that dehalogenation of iodinated IgM MAbs is a serious problem. We therefore suggest that internally labelled MAbs should be used and that further investigations should be carried out in a syngenic rat model, since this probably reflects the clinical situation better than the nude mouse model.

Original language	English
Pages (from-to)	379-383
Journal	Acta Oncologica
Volume	30
Issue number	3
DOIs	https://doi.org/10.3109/02841869109092390
Publication status	Published - 1991

Subject classification (UKÄ)

Cancer and Oncology

Free keywords

Monoclonal antibodies
colon carcinoma
rat

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3109/02841869109092390

Cite this

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title = "Radio-iodinated and internally labelled (35S) IgM monoclonal antibodies in a syngenic rat model",

abstract = "To simulate the human situation concerning human monoclonal antibodies (MAbs), we have introduced a new syngenic rat model with an implanted rat colon carcinoma. Rat IgM MAbs (10B12), labelled by the chloramine-T method with 125I or internally with 35S, were injected intravenously into the rats and the biodistribution was studied for 8 days. The radioactivity uptake in the tumours of the 35S label was higher than that of the 125I label and the retention of 35S in the tumours gave tumour/blood ratios 8 times higher than those of 125I at 48 and 96 h after injection. In this model we have shown that dehalogenation of iodinated IgM MAbs is a serious problem. We therefore suggest that internally labelled MAbs should be used and that further investigations should be carried out in a syngenic rat model, since this probably reflects the clinical situation better than the nude mouse model.",

keywords = "Monoclonal antibodies, colon carcinoma, rat",

author = "Q Wanying and T Brodin and Crister Ceberg and Christian Ingvar and Kristina Norrgren and Sjogren, {H O} and Sven-Erik Strand",

year = "1991",

doi = "10.3109/02841869109092390",

language = "English",

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pages = "379--383",

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TY - JOUR

T1 - Radio-iodinated and internally labelled (35S) IgM monoclonal antibodies in a syngenic rat model

AU - Wanying, Q

AU - Brodin, T

AU - Ceberg, Crister

AU - Ingvar, Christian

AU - Norrgren, Kristina

AU - Sjogren, H O

AU - Strand, Sven-Erik

PY - 1991

Y1 - 1991

N2 - To simulate the human situation concerning human monoclonal antibodies (MAbs), we have introduced a new syngenic rat model with an implanted rat colon carcinoma. Rat IgM MAbs (10B12), labelled by the chloramine-T method with 125I or internally with 35S, were injected intravenously into the rats and the biodistribution was studied for 8 days. The radioactivity uptake in the tumours of the 35S label was higher than that of the 125I label and the retention of 35S in the tumours gave tumour/blood ratios 8 times higher than those of 125I at 48 and 96 h after injection. In this model we have shown that dehalogenation of iodinated IgM MAbs is a serious problem. We therefore suggest that internally labelled MAbs should be used and that further investigations should be carried out in a syngenic rat model, since this probably reflects the clinical situation better than the nude mouse model.

AB - To simulate the human situation concerning human monoclonal antibodies (MAbs), we have introduced a new syngenic rat model with an implanted rat colon carcinoma. Rat IgM MAbs (10B12), labelled by the chloramine-T method with 125I or internally with 35S, were injected intravenously into the rats and the biodistribution was studied for 8 days. The radioactivity uptake in the tumours of the 35S label was higher than that of the 125I label and the retention of 35S in the tumours gave tumour/blood ratios 8 times higher than those of 125I at 48 and 96 h after injection. In this model we have shown that dehalogenation of iodinated IgM MAbs is a serious problem. We therefore suggest that internally labelled MAbs should be used and that further investigations should be carried out in a syngenic rat model, since this probably reflects the clinical situation better than the nude mouse model.

KW - Monoclonal antibodies

KW - colon carcinoma

KW - rat

U2 - 10.3109/02841869109092390

DO - 10.3109/02841869109092390

M3 - Article

SN - 1651-226X

VL - 30

SP - 379

EP - 383

JO - Acta Oncologica

JF - Acta Oncologica

IS - 3

ER -

Radio-iodinated and internally labelled (35S) IgM monoclonal antibodies in a syngenic rat model

Abstract

Subject classification (UKÄ)

Free keywords

UN SDGs

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