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Rapid fabrication of PMMA/PET-E/PMMA for thermoplastic microfluidic membrane devices

Henrik Persson, Siwan Park, Michael Mohan, Edmond Young, Craig A. Simmons

    Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceedingpeer-review

    Abstract

    We present a method to rapidly fabricate a large number of thermoplastic microfluidic systems where horizontal microchannels are separated by a porous membrane for modelling biological barriers. We optimize and combine solvent bonding, milling and laser cutting to fabricate in excess of 100 devices in two days. The optimized protocol relies on a commercial solvent blend, retention grooves for solvent distribution, and force application to create strong, leak-free bonds. The devices are evaluated for suitability for modelling biological barriers by culturing endothelial cells and measuring permeability using a plate reader.

    Original languageEnglish
    Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
    PublisherChemical and Biological Microsystems Society
    Pages1666-1667
    Number of pages2
    ISBN (Electronic)9781733419000
    Publication statusPublished - 2019
    Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
    Duration: 2019 Oct 272019 Oct 31

    Publication series

    Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

    Conference

    Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
    Country/TerritorySwitzerland
    CityBasel
    Period2019/10/272019/10/31

    Subject classification (UKÄ)

    • Medical Laboratory Technologies

    Free keywords

    • Laser cutting
    • Milling
    • Organ-on-a-chip
    • PET
    • PMMA
    • Solvent bonding
    • Thermoplastics

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