Rapid fragmentation of the endoplasmic reticulum in cortical neurons of the mouse brain in situ following cardiac arrest.

Krzysztof Kucharz; Tadeusz Wieloch; Håkan Toresson

doi:10.1038/jcbfm.2011.37

Rapid fragmentation of the endoplasmic reticulum in cortical neurons of the mouse brain in situ following cardiac arrest.

Krzysztof Kucharz, Tadeusz Wieloch, Håkan Toresson

Research output: Contribution to journal › Article › peer-review

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Abstract

Neuronal endoplasmic reticulum (ER), continuous from soma to dendritic spines, undergoes rapid fragmentation in response to N-methyl-D-aspartate (NMDA) receptor stimulation in hippocampal slices and neuronal primary cultures. Here, we show that ER fragments in the mouse brain following cardiac arrest (CA) induced brain ischemia. The ER structure was assessed in vivo in cortical pyramidal neurons in transgenic mice expressing ER-targeted GFP using two-photon laser scanning microscopy with fluorescence recovery after photobleaching (FRAP). Endoplasmic reticulum fragmentation occurred 1 to 2 minutes after CA and once induced, fragmentation was rapid (<15 seconds). We propose that acute ER fragmentation may be a protective response against severe ischemic stress.Journal of Cerebral Blood Flow & Metabolism advance online publication, 6 April 2011; doi:10.1038/jcbfm.2011.37.

Original language	English
Pages (from-to)	1663-1667
Journal	Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
Volume	31
DOIs	https://doi.org/10.1038/jcbfm.2011.37
Publication status	Published - 2011

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Clinical Memory Research Unit (013242610), Laboratory for Experimental Brain Research (013041000)

Subject classification (UKÄ)

Cardiology and Cardiovascular Disease

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http://www.ncbi.nlm.nih.gov/pubmed/21468089?dopt=Abstract

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title = "Rapid fragmentation of the endoplasmic reticulum in cortical neurons of the mouse brain in situ following cardiac arrest.",

abstract = "Neuronal endoplasmic reticulum (ER), continuous from soma to dendritic spines, undergoes rapid fragmentation in response to N-methyl-D-aspartate (NMDA) receptor stimulation in hippocampal slices and neuronal primary cultures. Here, we show that ER fragments in the mouse brain following cardiac arrest (CA) induced brain ischemia. The ER structure was assessed in vivo in cortical pyramidal neurons in transgenic mice expressing ER-targeted GFP using two-photon laser scanning microscopy with fluorescence recovery after photobleaching (FRAP). Endoplasmic reticulum fragmentation occurred 1 to 2 minutes after CA and once induced, fragmentation was rapid (<15 seconds). We propose that acute ER fragmentation may be a protective response against severe ischemic stress.Journal of Cerebral Blood Flow & Metabolism advance online publication, 6 April 2011; doi:10.1038/jcbfm.2011.37.",

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Rapid fragmentation of the endoplasmic reticulum in cortical neurons of the mouse brain in situ following cardiac arrest. / Kucharz, Krzysztof; Wieloch, Tadeusz ; Toresson, Håkan.
In: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, Vol. 31, 2011, p. 1663-1667.

Research output: Contribution to journal › Article › peer-review

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AU - Toresson, Håkan

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Clinical Memory Research Unit (013242610), Laboratory for Experimental Brain Research (013041000)

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Rapid fragmentation of the endoplasmic reticulum in cortical neurons of the mouse brain in situ following cardiac arrest.

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