Rapid in vitro and in vivo Evaluation of Antimicrobial Formulations Using Bioluminescent Pathogenic Bacteria

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Abstract

Basic and translational research needs rapid methods to test antimicrobial formulations. Bioluminescent bacteria and advanced imaging systems capable of acquiring bioluminescence enable us to quickly and longitudinally evaluate the efficacy of antimicrobials. Conventional approaches, such as radial diffusion and viable count assays, are time-consuming and do not allow for longitudinal analysis. Bioluminescence imaging is sensitive and gives vital spatial and temporal information on the infection status in the body. Here, using bioluminescent Pseudomonas aeruginosa, we describe an in vitro and an in vivo approach to rapidly evaluate the antimicrobial efficacy of the host-defense peptide TCP-25.

Original languageEnglish
Article numbere4302
JournalBio-protocol
Volume12
Issue number2
DOIs
Publication statusPublished - 2022 Jan

Subject classification (UKÄ)

  • Infectious Medicine

Keywords

  • Antimicrobials
  • Bioluminescent
  • In vivo imaging
  • In vivo models
  • Infection

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