Reagentless Amperometric Biosensor For Nadh Detection

Laura Muresan, Mihaela Nistor, Elisabeth Csöregi, Catalin Popescu

Research output: Contribution to journalArticlepeer-review


A sensitive and selective amperometric biosensor, based on NADH-oxidase (NADOx) working in tandem with horseradish peroxidase (HRP), which is electrically connected to graphite electrode via Os-redox polymer (RP), was developed. Two designs were considered for the biosensor optimization: (i) the first one, [G/HRP-NADOx], exploited the direct electron transfer between HRP and the graphite electrode (G); (ii) the second one, [G/RP-HR-P-NADOx], used R-P as mediator. In both cases the enzyme matrix was cross-linked using poly(ethylenglycol) diglycidyl ether. The biosensors sensitivities, estimated as the slopes of linear ranges from the calibration curves, recorded in a single line flow injection setup, showed that G/RP-HRP-NADOx electrode (1.62 +/- 0.05 mA M-1) presents a higher efficiency than G/HRP-NADOx electrode (0.30 +/- 0.03 mA M-1).
Original languageEnglish
Pages (from-to)755-760
JournalRevue Roumaine de Chimie
Issue number9
Publication statusPublished - 2009

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Biotechnology (LTH) (011001037), Analytical Chemistry (S/LTH) (011001004)

Subject classification (UKÄ)

  • Analytical Chemistry
  • Industrial Biotechnology

Free keywords

  • NADH detection
  • amperometric biosensors
  • NADOx
  • Os-redox polymer


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