Real-time PCR analysis for blood cell lineage specific markers.

Louise Edvardsson; Tor Olofsson

doi:10.1007/978-1-59745-553-4_21

Real-time PCR analysis for blood cell lineage specific markers.

Louise Edvardsson, Tor Olofsson

Division of Hematology and Clinical Immunology

Research output: Contribution to journal › Article › peer-review

Abstract

We here describe the methods for the isolation of distinct hematopoietic subpopulations, as defined by their immune phenotype by fluorescence-activated cell sorting, and how these cells can be analyzed even at a single-cell level for the gene expression of a number of transcription factors and other differentiation markers.

Original language	English
Pages (from-to)	313-322
Journal	Methods in Molecular Biology
Volume	496
DOIs	https://doi.org/10.1007/978-1-59745-553-4_21
Publication status	Published - 2009

Subject classification (UKÄ)

Hematology

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10.1007/978-1-59745-553-4_21

http://www.ncbi.nlm.nih.gov/pubmed/18839120?dopt=Abstract

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title = "Real-time PCR analysis for blood cell lineage specific markers.",

abstract = "We here describe the methods for the isolation of distinct hematopoietic subpopulations, as defined by their immune phenotype by fluorescence-activated cell sorting, and how these cells can be analyzed even at a single-cell level for the gene expression of a number of transcription factors and other differentiation markers.",

author = "Louise Edvardsson and Tor Olofsson",

year = "2009",

doi = "10.1007/978-1-59745-553-4_21",

language = "English",

volume = "496",

pages = "313--322",

journal = "Methods in Molecular Biology",

issn = "1940-6029",

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AU - Olofsson, Tor

PY - 2009

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N2 - We here describe the methods for the isolation of distinct hematopoietic subpopulations, as defined by their immune phenotype by fluorescence-activated cell sorting, and how these cells can be analyzed even at a single-cell level for the gene expression of a number of transcription factors and other differentiation markers.

AB - We here describe the methods for the isolation of distinct hematopoietic subpopulations, as defined by their immune phenotype by fluorescence-activated cell sorting, and how these cells can be analyzed even at a single-cell level for the gene expression of a number of transcription factors and other differentiation markers.

U2 - 10.1007/978-1-59745-553-4_21

DO - 10.1007/978-1-59745-553-4_21

M3 - Article

SN - 1940-6029

VL - 496

SP - 313

EP - 322

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Real-time PCR analysis for blood cell lineage specific markers.

Abstract

Subject classification (UKÄ)

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