Recombinant Protein Production Using the Baculovirus Expression Vector System (BEVS)

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Abstract

The baculovirus expression vector system (BEVS) is one of the most popular eukaryotic systems for recombinant protein production. The focus of our protein production platform is the provision of recombinant proteins for research use, where generally only small quantities are required, in the range of tens of micrograms to a few hundred milligrams. Here, we present methods that reflect our standard operating procedures and setup to be able to frequently, and often repeatedly, produce many different types of proteins.

Original languageEnglish
Title of host publicationAdvanced Methods in Structural Biology
EditorsAngela Sousa, Luis Passarinha
PublisherHumana Press
Pages55-77
Volume2652
ISBN (Electronic)978-1-0716-3147-8
ISBN (Print)978-1-0716-3146-1
DOIs
Publication statusPublished - 2023

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherSpringer
ISSN (Print)1940-6029

Bibliographical note

© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Subject classification (UKÄ)

  • Structural Biology
  • Biochemistry and Molecular Biology

Free keywords

  • Baculoviridae/genetics
  • Genetic Vectors
  • Recombinant Proteins/metabolism

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