Regional brain-derived neurotrophic factor mRNA and protein levels following transient forebrain ischemia in the rat

Zaal Kokaia; H Nawa; H Uchino; Eskil Elmer; M Kokaia; J Carnahan; M L Smith; B K Siesjo; O Lindvall

doi:10.1016/0169-328X(96)00002-2

Regional brain-derived neurotrophic factor mRNA and protein levels following transient forebrain ischemia in the rat

Zaal Kokaia, H Nawa, H Uchino, Eskil Elmer, M Kokaia, J Carnahan, M L Smith, B K Siesjo, O Lindvall

Research output: Contribution to journal › Article › peer-review

Abstract

Levels of BDNF mRNA and protein were measured in the rat brain using in situ hybridization and a two-site enzyme immunoassay. Under basal conditions, the highest BDNF concentration was found in the dentate gyrus (88 ng/g), while the levels in CA3 (50 ng/g), CA1 (18 ng/g) and parietal cortex (8 ng/g) were markedly lower. Following 10 min of forebrain ischemia, BDNF protein increased transiently in the dentate gyrus (to 124% of control at 6 h after the insult) and CA3 region (to 131% of control, at 1 week after the insult). In CA1 and parietal cortex, BDNF protein decreased to 73-75% of control at 24 h. In contrast, BDNF mRNA expression in dentate granule cells and CA3 pyramidal layer was transiently elevated to 287 and 293% of control, respectively, at 2 h, whereas no change was detected in CA1 or neocortex. The regional BDNF protein levels shown here correlate at least partly with regional differences in cellular resistance to ischemic damage, which is consistent with the hypothesis of a neuroprotective role of BDNF.

Original language	English
Pages (from-to)	139-144
Journal	Brain Research. Molecular Brain Research
Volume	38
Issue number	1
DOIs	https://doi.org/10.1016/0169-328X(96)00002-2
Publication status	Published - 1996

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Neurology, Lund (013027000), Laboratory for Experimental Brain Research (013041000)

Subject classification (UKÄ)

Neurology

Keywords

Rat
Brain damage
Hippocampus
Neurotrophins
Forebrain ischemia

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10.1016/0169-328X(96)00002-2

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title = "Regional brain-derived neurotrophic factor mRNA and protein levels following transient forebrain ischemia in the rat",

abstract = "Levels of BDNF mRNA and protein were measured in the rat brain using in situ hybridization and a two-site enzyme immunoassay. Under basal conditions, the highest BDNF concentration was found in the dentate gyrus (88 ng/g), while the levels in CA3 (50 ng/g), CA1 (18 ng/g) and parietal cortex (8 ng/g) were markedly lower. Following 10 min of forebrain ischemia, BDNF protein increased transiently in the dentate gyrus (to 124% of control at 6 h after the insult) and CA3 region (to 131% of control, at 1 week after the insult). In CA1 and parietal cortex, BDNF protein decreased to 73-75% of control at 24 h. In contrast, BDNF mRNA expression in dentate granule cells and CA3 pyramidal layer was transiently elevated to 287 and 293% of control, respectively, at 2 h, whereas no change was detected in CA1 or neocortex. The regional BDNF protein levels shown here correlate at least partly with regional differences in cellular resistance to ischemic damage, which is consistent with the hypothesis of a neuroprotective role of BDNF.",

keywords = "Rat, Brain damage, Hippocampus, Neurotrophins, Forebrain ischemia",

author = "Zaal Kokaia and H Nawa and H Uchino and Eskil Elmer and M Kokaia and J Carnahan and Smith, {M L} and Siesjo, {B K} and O Lindvall",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neurology, Lund (013027000), Laboratory for Experimental Brain Research (013041000)",

year = "1996",

doi = "10.1016/0169-328X(96)00002-2",

language = "English",

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pages = "139--144",

journal = "Brain Research",

issn = "1872-6240",

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T1 - Regional brain-derived neurotrophic factor mRNA and protein levels following transient forebrain ischemia in the rat

AU - Kokaia, Zaal

AU - Nawa, H

AU - Uchino, H

AU - Elmer, Eskil

AU - Kokaia, M

AU - Carnahan, J

AU - Smith, M L

AU - Siesjo, B K

AU - Lindvall, O

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neurology, Lund (013027000), Laboratory for Experimental Brain Research (013041000)

PY - 1996

Y1 - 1996

N2 - Levels of BDNF mRNA and protein were measured in the rat brain using in situ hybridization and a two-site enzyme immunoassay. Under basal conditions, the highest BDNF concentration was found in the dentate gyrus (88 ng/g), while the levels in CA3 (50 ng/g), CA1 (18 ng/g) and parietal cortex (8 ng/g) were markedly lower. Following 10 min of forebrain ischemia, BDNF protein increased transiently in the dentate gyrus (to 124% of control at 6 h after the insult) and CA3 region (to 131% of control, at 1 week after the insult). In CA1 and parietal cortex, BDNF protein decreased to 73-75% of control at 24 h. In contrast, BDNF mRNA expression in dentate granule cells and CA3 pyramidal layer was transiently elevated to 287 and 293% of control, respectively, at 2 h, whereas no change was detected in CA1 or neocortex. The regional BDNF protein levels shown here correlate at least partly with regional differences in cellular resistance to ischemic damage, which is consistent with the hypothesis of a neuroprotective role of BDNF.

AB - Levels of BDNF mRNA and protein were measured in the rat brain using in situ hybridization and a two-site enzyme immunoassay. Under basal conditions, the highest BDNF concentration was found in the dentate gyrus (88 ng/g), while the levels in CA3 (50 ng/g), CA1 (18 ng/g) and parietal cortex (8 ng/g) were markedly lower. Following 10 min of forebrain ischemia, BDNF protein increased transiently in the dentate gyrus (to 124% of control at 6 h after the insult) and CA3 region (to 131% of control, at 1 week after the insult). In CA1 and parietal cortex, BDNF protein decreased to 73-75% of control at 24 h. In contrast, BDNF mRNA expression in dentate granule cells and CA3 pyramidal layer was transiently elevated to 287 and 293% of control, respectively, at 2 h, whereas no change was detected in CA1 or neocortex. The regional BDNF protein levels shown here correlate at least partly with regional differences in cellular resistance to ischemic damage, which is consistent with the hypothesis of a neuroprotective role of BDNF.

KW - Rat

KW - Brain damage

KW - Hippocampus

KW - Neurotrophins

KW - Forebrain ischemia

U2 - 10.1016/0169-328X(96)00002-2

DO - 10.1016/0169-328X(96)00002-2

M3 - Article

VL - 38

SP - 139

EP - 144

JO - Brain Research

JF - Brain Research

SN - 1872-6240

IS - 1

ER -

Regional brain-derived neurotrophic factor mRNA and protein levels following transient forebrain ischemia in the rat

Abstract

Bibliographical note

Subject classification (UKÄ)

Keywords

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