Abstract
In this report we demonstrate an increase in the steady-state level of bone sialoprotein (BSP) mRNA in rat calvaria and a rat osteosarcoma cell line (ROS 17/2.8) after treatment with the synthetic glucocorticoid, dexamethasone. In contrast, 1.25-dihydroxyvitamin D3 reduced the amount of BSP mRNA in calvaria and inhibited the dexamethasone induction in ROS 17/2.8 cells. The increase in BSP mRNA is most likely due to an increase in the transcriptional rate. The stability of mRNA was unchanged after dexamethasone treatment with a half-life of approximately 5 h. Nuclear transcription experiments with nuclei isolated from ROS 17/2.8 cells showed an increased BSP mRNA synthesis in cells treated with dexamethasone.
| Original language | English |
|---|---|
| Pages (from-to) | 3183-3186 |
| Journal | Journal of Cell Biology |
| Volume | 109 |
| Issue number | 6 Pt 1 |
| Publication status | Published - 1989 |
Subject classification (UKÄ)
- Oto-rhino-laryngology
Free keywords
- Messenger: genetics
- Messenger: drug effects
- RNA
- Genes: drug effects
- Gene Expression Regulation: drug effects
- Dexamethasone: pharmacology
- Calcitriol: pharmacology
- Bone and Bones: drug effects
- Bone and Bones: metabolism
- Sialoglycoproteins: genetics
- Transcription
- Genetic: drug effects