Regulation of bone sialoprotein mRNA by steroid hormones.

Åke Oldberg, B Jirskog-Hed, Sara Axelsson, Dick Heinegård

Research output: Contribution to journalArticlepeer-review

Abstract

In this report we demonstrate an increase in the steady-state level of bone sialoprotein (BSP) mRNA in rat calvaria and a rat osteosarcoma cell line (ROS 17/2.8) after treatment with the synthetic glucocorticoid, dexamethasone. In contrast, 1.25-dihydroxyvitamin D3 reduced the amount of BSP mRNA in calvaria and inhibited the dexamethasone induction in ROS 17/2.8 cells. The increase in BSP mRNA is most likely due to an increase in the transcriptional rate. The stability of mRNA was unchanged after dexamethasone treatment with a half-life of approximately 5 h. Nuclear transcription experiments with nuclei isolated from ROS 17/2.8 cells showed an increased BSP mRNA synthesis in cells treated with dexamethasone.
Original languageEnglish
Pages (from-to)3183-3186
JournalJournal of Cell Biology
Volume109
Issue number6 Pt 1
Publication statusPublished - 1989

Subject classification (UKÄ)

  • Otorhinolaryngology

Free keywords

  • Messenger: genetics
  • Messenger: drug effects
  • RNA
  • Genes: drug effects
  • Gene Expression Regulation: drug effects
  • Dexamethasone: pharmacology
  • Calcitriol: pharmacology
  • Bone and Bones: drug effects
  • Bone and Bones: metabolism
  • Sialoglycoproteins: genetics
  • Transcription
  • Genetic: drug effects

Fingerprint

Dive into the research topics of 'Regulation of bone sialoprotein mRNA by steroid hormones.'. Together they form a unique fingerprint.

Cite this