Regulation of mitogen-induced lymphocyte DNA synthesis by human interferon of different origins

Håkan Miörner, Lars-Erik Landström, Ebba Larner, Inger Larsson, Erik Lundgren, Örjan Strannegård

Research output: Contribution to journalArticlepeer-review

Abstract

Human fibroblast and leukocyte interferons were found to suppress lymphocyte mitogenesis induced by optimal doses of phytohemagglutinin and concanavalin A. In certain situations (low doses of mitogen and/or low doses of interferon), however, interferon significantly enhanced mitogenesis. In experiments using varying concentrations of interferon, dose-response curves with different slopes were obtained for fibroblast and leukocyte interferons. The effect of interferon was apparently exerted during early stages of the lymphocyte cell cycle. There was no inhibitory effect of interferon if the lymphocytes were washed with medium before being exposed to mitogen. Interferon increased the binding of radiolabeled mitogens to cells. The results suggest that the immunological effects of interferon are consequences of actions on lymphoid cells. Fibroblast and leukocyte interferons seem to have different modes of action, or to bind differently to target cells. Possible mechanisms for the suppressive and enhancing effects of interferons on lymphoid cells are discussed.
Original languageEnglish
Pages (from-to)15-24
JournalCellular Immunology
Volume35
Issue number1
DOIs
Publication statusPublished - 1978

Subject classification (UKÄ)

  • Microbiology in the medical area

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