Abstract
The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [C-14]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [C-14]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed (CO2)-C-14 evolution and greatly reduced the flow of carbon from [C-14]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.
Original language | English |
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Pages (from-to) | 87-91 |
Journal | FEMS Microbiology Letters |
Volume | 121 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1994 |
Subject classification (UKÄ)
- Biological Sciences
Free keywords
- Amino acid respiration
- Aminooxyacetate
- [14C]alanine
- Ectomycorrhizal fungus
- Paxillus involutus
- TCA cycle