Restricted variable region gene usage and possible rheumatoid factor relationship among human monoclonal antibodies specific for the AD-1 epitope on cytomegalovirus glycoprotein B

Mats Ohlin, Henrik Owman, John D Rioux, Marianna M Newkirk, Carl Borrebaeck

Research output: Contribution to journalArticlepeer-review

Abstract

The nucleotide sequences of the variable region genes encoding five different human, high affinity antibodies, specific for the major neutralization determinant (AD-1) expressed by human cytomegalovirus glycoprotein B (gp58/116), have been determined. Three of the five heavy chain variable regions belonged to the small VHV-family, although they combined with a diverse set of light chains (V kappa IIIb, V lambda II and V lambda III). The other two antibodies belonged to VH-families III and IV. One of the VHV-family genes most likely originated from a previously unreported germline gene or allele, since it carries a nine nucleotide insert in framework 1. In addition, V lambda-genes showed variable homology (77-95%) to known germline sequences, while V kappa-genes showed high homology (approximately 98%) with their proposed germline origin. Despite the close homology of the V kappa IIIb-gene used to express one of the antibodies with its corresponding germline gene, the protein did not strongly express some idiotypes associated with this light chain family. There is, thus, no direct relation between the expression of these crossreactive idiotypes and the use of even modestly mutated light chains belonging to this V kappa-family, which has been implicated in the development of anti-idiotypic networks possibly inducing autoantibodies, such as rheumatoid factors.
Original languageEnglish
Pages (from-to)983-991
JournalMolecular Immunology
Volume31
Issue number13
DOIs
Publication statusPublished - 1994

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Department of Immunotechnology (011029300), Reconstructive Surgery (013240300)

Subject classification (UKÄ)

  • Immunology in the medical area

Free keywords

  • cytomegalovirus
  • glycoprotein B
  • human monoclonal antibody
  • rheumatoid factor
  • variable region gene

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