Ribosylurea accumulates in yeast urc4 mutants.

Olof Björnberg, M Vodnala, V Domkin, A Hofer, Anna Rasmussen, G Andersen, Jure Piskur

Research output: Contribution to journalArticlepeer-review

Abstract

Yeast Saccharomyces (Lachancea) kluyveri urc4 mutants, unable to grow on uracil, biotransformed (14)C(2)-uracil into two labeled compounds, as detected by high performance liquid chromatography (HPLC). These two compounds could also be obtained following organic synthesis of ribosylurea. This finding demonstrates that in the URC pyrimidine degradation pathway, the opening of the uracil ring takes place when uracil is attached to the ribose moiety. Ribosylurea has not been reported in the cell metabolism before and the two observed compounds likely represent an equilibrium mixture of the pyranosyl and furanosyl forms.
Original languageEnglish
Pages (from-to)433-437
JournalNucleosides, Nucleotides & Nucleic Acids
Volume29
Issue number4-6
DOIs
Publication statusPublished - 2010

Subject classification (UKÄ)

  • Biological Sciences

Fingerprint

Dive into the research topics of 'Ribosylurea accumulates in yeast urc4 mutants.'. Together they form a unique fingerprint.

Cite this