Ribosylurea accumulates in yeast urc4 mutants.

Olof Björnberg; M Vodnala; V Domkin; A Hofer; Anna Rasmussen; G Andersen; Jure Piskur

doi:10.1080/15257771003741265

Ribosylurea accumulates in yeast urc4 mutants.

Olof Björnberg, M Vodnala, V Domkin, A Hofer, Anna Rasmussen, G Andersen, Jure Piskur

Department of Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Yeast Saccharomyces (Lachancea) kluyveri urc4 mutants, unable to grow on uracil, biotransformed (14)C(2)-uracil into two labeled compounds, as detected by high performance liquid chromatography (HPLC). These two compounds could also be obtained following organic synthesis of ribosylurea. This finding demonstrates that in the URC pyrimidine degradation pathway, the opening of the uracil ring takes place when uracil is attached to the ribose moiety. Ribosylurea has not been reported in the cell metabolism before and the two observed compounds likely represent an equilibrium mixture of the pyranosyl and furanosyl forms.

Original language	English
Pages (from-to)	433-437
Journal	Nucleosides, Nucleotides & Nucleic Acids
Volume	29
Issue number	4-6
DOIs	https://doi.org/10.1080/15257771003741265
Publication status	Published - 2010

Subject classification (UKÄ)

Biological Sciences

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10.1080/15257771003741265

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title = "Ribosylurea accumulates in yeast urc4 mutants.",

abstract = "Yeast Saccharomyces (Lachancea) kluyveri urc4 mutants, unable to grow on uracil, biotransformed (14)C(2)-uracil into two labeled compounds, as detected by high performance liquid chromatography (HPLC). These two compounds could also be obtained following organic synthesis of ribosylurea. This finding demonstrates that in the URC pyrimidine degradation pathway, the opening of the uracil ring takes place when uracil is attached to the ribose moiety. Ribosylurea has not been reported in the cell metabolism before and the two observed compounds likely represent an equilibrium mixture of the pyranosyl and furanosyl forms.",

author = "Olof Bj{\"o}rnberg and M Vodnala and V Domkin and A Hofer and Anna Rasmussen and G Andersen and Jure Piskur",

year = "2010",

doi = "10.1080/15257771003741265",

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pages = "433--437",

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T1 - Ribosylurea accumulates in yeast urc4 mutants.

AU - Björnberg, Olof

AU - Vodnala, M

AU - Domkin, V

AU - Hofer, A

AU - Rasmussen, Anna

AU - Andersen, G

AU - Piskur, Jure

PY - 2010

Y1 - 2010

N2 - Yeast Saccharomyces (Lachancea) kluyveri urc4 mutants, unable to grow on uracil, biotransformed (14)C(2)-uracil into two labeled compounds, as detected by high performance liquid chromatography (HPLC). These two compounds could also be obtained following organic synthesis of ribosylurea. This finding demonstrates that in the URC pyrimidine degradation pathway, the opening of the uracil ring takes place when uracil is attached to the ribose moiety. Ribosylurea has not been reported in the cell metabolism before and the two observed compounds likely represent an equilibrium mixture of the pyranosyl and furanosyl forms.

AB - Yeast Saccharomyces (Lachancea) kluyveri urc4 mutants, unable to grow on uracil, biotransformed (14)C(2)-uracil into two labeled compounds, as detected by high performance liquid chromatography (HPLC). These two compounds could also be obtained following organic synthesis of ribosylurea. This finding demonstrates that in the URC pyrimidine degradation pathway, the opening of the uracil ring takes place when uracil is attached to the ribose moiety. Ribosylurea has not been reported in the cell metabolism before and the two observed compounds likely represent an equilibrium mixture of the pyranosyl and furanosyl forms.

U2 - 10.1080/15257771003741265

DO - 10.1080/15257771003741265

M3 - Article

C2 - 20544532

SN - 1525-7770

VL - 29

SP - 433

EP - 437

JO - Nucleosides, Nucleotides & Nucleic Acids

JF - Nucleosides, Nucleotides & Nucleic Acids

IS - 4-6

ER -

Ribosylurea accumulates in yeast urc4 mutants.

Abstract

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