RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.

Aurelie Baudet; Christine Karlsson; Mehrnaz Safaee Talkhoncheh; Roman Galeev; Mattias Magnusson; Jonas Larsson

doi:10.1182/blood-2012-01-403949

RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.

Aurelie Baudet, Christine Karlsson, Mehrnaz Safaee Talkhoncheh, Roman Galeev, Mattias Magnusson, Jonas Larsson

Research output: Contribution to journal › Article › peer-review

Abstract

We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.

Original language	English
Pages (from-to)	6255-6258
Journal	Blood
Volume	119
Issue number	26
DOIs	https://doi.org/10.1182/blood-2012-01-403949
Publication status	Published - 2012

Subject classification (UKÄ)

Hematology

Access to Document

10.1182/blood-2012-01-403949

http://www.ncbi.nlm.nih.gov/pubmed/22555972?dopt=Abstract

3 Doctoral Thesis (compilation)

Ex Vivo Expansion of Human Hematopoietic Stem Cells
Safaee Talkhoncheh, M., 2019, Lund: Lund University: Faculty of Medicine. 100 p.
Research output: Thesis › Doctoral Thesis (compilation)
Identification of intrinsic regulators in normal and malignant human hematopoiesis. An RNA-interference approach.
Galeev, R., 2018, Lund: Lund University: Faculty of Medicine. 127 p.
Research output: Thesis › Doctoral Thesis (compilation)

Open Access
File
Defining Regulators of Human Hematopoietic Stem Cells
Karlsson, C., 2013, Division of Molecular Medicine and Gene Therapy. 98 p.
Research output: Thesis › Doctoral Thesis (compilation)

Cite this

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title = "RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.",

abstract = "We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.",

author = "Aurelie Baudet and Christine Karlsson and {Safaee Talkhoncheh}, Mehrnaz and Roman Galeev and Mattias Magnusson and Jonas Larsson",

year = "2012",

doi = "10.1182/blood-2012-01-403949",

language = "English",

volume = "119",

pages = "6255--6258",

journal = "Blood",

issn = "1528-0020",

publisher = "American Society of Hematology",

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T1 - RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.

AU - Baudet, Aurelie

AU - Karlsson, Christine

AU - Safaee Talkhoncheh, Mehrnaz

AU - Galeev, Roman

AU - Magnusson, Mattias

AU - Larsson, Jonas

PY - 2012

Y1 - 2012

N2 - We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.

AB - We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.

U2 - 10.1182/blood-2012-01-403949

DO - 10.1182/blood-2012-01-403949

M3 - Article

C2 - 22555972

SN - 1528-0020

VL - 119

SP - 6255

EP - 6258

JO - Blood

JF - Blood

IS - 26

ER -

RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.

Abstract

Subject classification (UKÄ)

Access to Document

Fingerprint

Research output

Ex Vivo Expansion of Human Hematopoietic Stem Cells

Identification of intrinsic regulators in normal and malignant human hematopoiesis. An RNA-interference approach.

Defining Regulators of Human Hematopoietic Stem Cells

Cite this