Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout

Lars Wallman, Simon Ekström, Mattias Magnusson, Gunnar Bolmsjö, Magnus Olsson, Johan Nilsson, György Marko-Varga, Thomas Laurell

Research output: Contribution to journalArticlepeer-review


A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.
Original languageEnglish
Pages (from-to)3147-3153
JournalMeasurement Science & Technology
Issue number12
Publication statusPublished - 2006

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Biomedical Engineering (011200011), Robotics (ceased) (LUR000026), Analytical Chemistry (S/LTH) (011001004), Departments at LTH (011200000)

Subject classification (UKÄ)

  • Analytical Chemistry
  • Medical Engineering

Free keywords

  • piezodispensing
  • capillary force
  • microfluidics
  • mass spectrometry
  • microchip
  • robotic
  • automation
  • protein analysis
  • proteomics
  • solid phase extraction


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