Abstract
A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.
| Original language | English |
|---|---|
| Pages (from-to) | 3147-3153 |
| Journal | Measurement Science & Technology |
| Volume | 17 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2006 |
Bibliographical note
The information about affiliations in this record was updated in December 2015.The record was previously connected to the following departments: Biomedical Engineering (011200011), Robotics (ceased) (LUR000026), Analytical Chemistry (S/LTH) (011001004), Departments at LTH (011200000)
Subject classification (UKÄ)
- Analytical Chemistry
- Medical Engineering
Free keywords
- piezodispensing
- capillary force
- microfluidics
- MALDI
- mass spectrometry
- microchip
- robotic
- MALDI-TOF MS
- automation
- protein analysis
- proteomics
- solid phase extraction