Abstract
Rrp6 is an exoribonuclease involved in the quality control of mRNA biogenesis. We have analyzed the association of Rrp6 with the Balbiani ring pre-mRNPs of Chironomus tentans to obtain insight into the role of Rrp6 in splicing surveillance. Rrp6 is recruited to transcribed genes and its distribution along the genes does not correlate with the positions of exons and introns. In the nucleoplasm, Rrp6 is bound to both unspliced and spliced transcripts. Rrp6 is released from the mRNPs in the vicinity of the nuclear pore before nucleo-cytoplasmic translocation. We show that Rrp6 is associated with newly synthesized transcripts during all the nuclear steps of gene expression and is associated with the transcripts independently of their splicing status. These observations suggest that the quality control of pre-mRNA splicing is not based on the selective recruitment of the exoribonuclease Rrp6 to unprocessed mRNAs.
Original language | English |
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Pages (from-to) | 1466-74 |
Number of pages | 9 |
Journal | RNA |
Volume | 18 |
Issue number | 8 |
DOIs | |
Publication status | Published - 2012 Aug |
Externally published | Yes |
Free keywords
- Animals
- Blotting, Western
- Cell Nucleus
- Cells, Cultured
- Chironomidae
- Chromatin Immunoprecipitation
- Cytoplasm
- Drosophila Proteins
- Drosophila melanogaster
- Exons
- Exosome Multienzyme Ribonuclease Complex
- Fluorescent Antibody Technique
- Introns
- Nuclear Pore
- Nuclear Proteins
- RNA Precursors
- RNA Splicing
- RNA, Messenger
- Rabbits
- Ribonucleoproteins
- Salivary Glands
- Transcription, Genetic
- Journal Article
- Research Support, Non-U.S. Gov't