Sampling and Selective Detection of Peptides and Proteins Emphazing Microdialysis,Bio-recognition Assays and MALDI-TOF MS

Sven Kjellström

Research output: ThesisDoctoral Thesis (compilation)

Abstract

The use of microdialysis sampling and flow immunochemical detection as well as MALDI-TOF MS for peptide and protein analysis is described. Sampling of peptides and smaller proteins was investigated with different membranes. Cyclodextrines additives and an on-line push-pull microdialysis sampling method was used with the result of a higher sampling efficiency compared to conventional microdialysis sampling.

Real-time flow immuno assays systems were developed for the quantification of Interleukin-8 and Interleukin10 in cell samples. Optimisation with regards to affinity support selection as well as carrier buffer composition were performed.

Connection of capillary LC with MALDI-TOF MS was made utilising an m-dispenser technique enabling sample handling of picoliter volumes. A novel sample preparation technique for the uniform coating of MALDI targets was developed.
Original languageEnglish
QualificationDoctor
Awarding Institution
  • Centre for Analysis and Synthesis
Supervisors/Advisors
  • [unknown], [unknown], Supervisor, External person
Award date2000 Nov 17
Publisher
Print ISBNs91-7874-077-0
Publication statusPublished - 2000

Bibliographical note

Defence details

Date: 2000-11-17
Time: 13:15
Place: Lecture Hall B Chemical Center

External reviewer(s)

Name: Andren, Per
Title: [unknown]
Affiliation: [unknown]

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The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)

Subject classification (UKÄ)

  • Analytical Chemistry

Keywords

  • cyclodextrines
  • protein identification
  • m-dispenser
  • push-pull microdialysis
  • microdialysis sampling
  • MALDI-TOF MS
  • Interleukin-8
  • Flow immuno assays
  • Interleukin-10
  • Analytical chemistry
  • Analytisk kemi

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